Protein accumulation in lung lavage fluid following ozone exposure

Journal Article · Wed Dec 01 00:00:00 EST 1982 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/0013-9351(82)90039-1· OSTI ID:5238051

Hu, P C ^[1]; Miller, F J; Daniels, M J; Hatch, G E; Graham, J A; Gardner, D E; Selgrade, M K

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1. Northrop Services, Inc., Research Triangle Park, NC

Accumulation of protein in lung lavage fluid was used as an indicator of pulmonary damage following exposure of guinea pigs to O/sub 3/. Exposure of animals to 510, 1000, or 1960 ..mu..g/m/sup 3/ (0.26, 0.51, or 1.0 ppm) of O/sub 3/ for 72 hr resulted in significantly elevated levels of lavage fluid protein when compared to that of air controls. This effect was not observed in animals exposed to 196 ..mu..g O/sub 3//m/sup 3/ (0.10 ppm). When exposure time was reduced to 3 hr, the O/sub 3/-induced protein accumulation in lavage fluids obtained from animals exposed to O/sub 3/ ranging from 510 to 1470 ..mu..g O/sub 3//m/sup 3/ (0.26-0.75 ppm) and a dose relationship between the amount of protein accumulation in the lung and the concentration of O/sub 3/ to which the animals were exposed was observed. Vitamin C deficiency did not enhance this O/sub 3/-induced lesion in guinea pigs. The dose relationship has also been confirmed by polyacrylamide gel electrophoresis of the lavage fluids. Lung lavage fluid protein content in animals exposed to 353 ..mu..g O/sub 3//m/sup 3/ (0.18 ppm) for 8 hr/day for 5 or 10 consecutive days was not different from that of air controls.

• OSTI ID: 5238051
• Journal Information: Environ. Res.; (United States), Vol. 29:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BODY FLUIDS
ELECTROPHORESIS
OZONE
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
GUINEA PIGS
LAVAGE
LUNGS
PROTEINS
ANIMALS
BIOLOGICAL MATERIALS
BODY
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
ORGANS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
560306* - Chemicals Metabolism & Toxicology- Man- (-1987)