Enhancing effects of gamma interferon on phagocytic cell association with and killing of Trypanosoma cruzi
Results are reported from a study of the influence gamma interferon (GIFN) and interleukin 2 (IL2) have on the capability of P388D1 cells and mouse resident peritoneal macrophages (MPM) to attach to the blood-resident parasites Trypanosoma cruzi and kill them. Cultures of trypomastigote forms of the Tulahuen strain of T. cruzi grown in bovine serum were introduced into peritoneal cells of mice, along with P388D1 cells incubated with GIFN, IL2 and both. Control cells were also maintained. Statistical analysis were then performed on data on counts of the number of dead T. Cruzi cells. The GIFN enhanced the interaction of MPM and P388D1 cells with the surface of T. Cruzi, provided the interaction was given over 12 hr to take place. A depression of the cytotoxicity of P388D1 cells was attributed to mediation by H2O2, an effect partially offset by incubation with the lymphokine GIFN. 23 references.
- Research Organization:
- Michigan State Univ., East Lansing; Louisville Univ., KY; DNAX Research Institute, Palo Alto, CA
- OSTI ID:
- 5232805
- Journal Information:
- Infect. Immun.; (United States), Vol. 49
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
INFECTIOUS DISEASES
IMMUNITY
ANIMAL CELLS
DRUGS
INTERFERON
MACROPHAGES
MICE
PARASITES
TRYPANOSOMA
ANIMALS
CONNECTIVE TISSUE CELLS
DISEASES
GROWTH FACTORS
LYMPHOKINES
MAMMALS
MASTIGOPHORA
MITOGENS
ORGANIC COMPOUNDS
PHAGOCYTES
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
550600* - Medicine
550300 - Cytology