Quantitative and molecular analyses of radiation-induced mutation in AS52 cells
pSV2gpt-Transformed and wild-type Chinese hamster ovary (CHO) cell lines have been used to study radiation-induced mutation at the molecular level. The transformant, designated AS52, was constructed from a hypoxanthine-guanine phosphoribosyl transferase (HPRT)-deficient CHO cell line and contains a single, functional copy of the Escherichia coli xanthine-guanine phosphoribosyl transferase (XPRT) gene (gpt) stably integrated into the Chinese hamster genome. AS52 and wild-type CHO-K1-BH4 cells exhibit similar cytotoxic responses to uv light and X rays; however, significant differences occur in mutation induction at the gpt and hprt loci. A number of HPRT and XPRT mutants which arose following irradiation were analyzed by Southern-blot hybridization. Most XPRT (21/26) and all HPRT (23/23) mutants induced by uv light exhibited hybridization patterns indistinguishable from their parental cell lines. In contrast, all XPRT (26/26) and most HPRT mutants (15/21) induced by X irradiation contained deletion mutations affecting some or all of the gpt and hprt loci, respectively. These results indicate that X rays induce predominantly deletion mutations, while uv light is likely to induce point mutations at both loci.
- Research Organization:
- Univ. of Tennessee-Oak Ridge Graduate School of Biomedical Sciences
- OSTI ID:
- 5231252
- Journal Information:
- Radiat. Res.; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHO CELLS
GENETIC RADIATION EFFECTS
MUTATIONS
TRANSFERASES
ULTRAVIOLET RADIATION
X RADIATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
ELECTROMAGNETIC RADIATION
ENZYMES
GENETIC EFFECTS
IONIZING RADIATIONS
RADIATION EFFECTS
RADIATIONS
560121* - Radiation Effects on Cells- External Source- (-1987)