Use of tritiated prostaglandins in metabolism studies. I: Evaluation of the kinetic isotope effect in the prostaglandin dehydrogenase reactions

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(86)90111-5· OSTI ID:5230718

Moussard, C; Alber, D; Perruche, C; Henry, J C

Although numerous data exist concerning tritium kinetic isotope effect in enzymic reactions, little is related to the metabolism of tritiated prostaglandins. The present study reports an evaluation of the kinetic isotope effect which occurs during the oxidation of 15-hydroxyl group of tritium-labeled prostaglandins E2 and F2 alpha by the 15-hydroxyprostaglandin dehydrogenase and during the oxidation of 9-hydroxyl group of tritium-labeled prostaglandin F2 alpha by the 9-hydroxyprostaglandin dehydrogenase. The large kinetic isotope effect tends to limit the validity of the dehydrogenase assay using tritium-labeled prostaglandins as substrate. However these assays can be considered to be an indication of relative enzyme activity.

• Research Organization: Universite de Franche-Comte, Besancon, France
• OSTI ID: 5230718
• Journal Information: Prostaglandins; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PROSTAGLANDINS
METABOLISM
TRITIUM COMPOUNDS
ISOTOPE EFFECTS
ENZYME ACTIVITY
GUINEA PIGS
IN VITRO
OXIDOREDUCTASES
PLACENTA
PREGNANCY
RADIONUCLIDE KINETICS
RATS
ANIMALS
ENZYMES
FETAL MEMBRANES
LABELLED COMPOUNDS
MAMMALS
MEMBRANES
RODENTS
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)