Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism

Mayer, D; Branscheid, D

doi:10.1080/15287399209531614

Title: Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism

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Abstract

Exposure of cultures of human lung fibroblasts to 0.5 ppm ozone for 20 h resulted in a significant increase in cellular mortality by 29%; after exposure to 2.5 ppm ozone for 4 h, the increase amounted to 74%. A marked difference in sensitivity to ozone was observed between fibroblast lines from different individuals. This variability in resistance to ozone was more evident after exposure to 0.5 ppm ozone for 20 h, when compared with 2.5 ppm ozone for 4 h. In one fibroblast line, synthesis of hyaluronan was enhanced by exposure to 0.5 ppm ozone for 20 h. The concentrations of hyaluronan in culture media increased in experiments using different fibroblast cell lines, a phenomenon that was obvious both if cell numbers and combined protein concentrations of cells and media are selected as references for hyaluronan concentrations.

Authors:

Mayer, D; Branscheid, D ^[1]

Thoraxklinik Heidelberg-Rohrbach, Heidelberg, (West Germany)

Publication Date:: Wed Apr 01 00:00:00 EST 1992

OSTI Identifier:: 5227045

Resource Type:: Journal Article

Journal Name:: Journal of Toxicology and Environmental Health; (United States)

Additional Journal Information:: Journal Volume: 35:4; Journal ID: ISSN 0098-4108

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; FIBROBLASTS; CELL KILLING; HYALURONIC ACID; METABOLISM; OZONE; TOXICITY; CELL CULTURES; CULTURE MEDIA; LUNGS; MAN; PROTEINS; AMINES; ANIMAL CELLS; ANIMALS; BODY; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; MAMMALS; MUCOPOLYSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; PRIMATES; RESPIRATORY SYSTEM; SACCHARIDES; SOMATIC CELLS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats


                    Mayer, D, and Branscheid, D. Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism.  United States: N. p., 1992. 
        Web.  doi:10.1080/15287399209531614.

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                    Mayer, D, & Branscheid, D. Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism.  United States.  https://doi.org/10.1080/15287399209531614

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                    Mayer, D, and Branscheid, D. 1992.  
        "Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism".  United States.  https://doi.org/10.1080/15287399209531614.

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@article{osti_5227045,

  title        = {Exposure of human lung fibroblasts to ozone: cell mortality and hyaluronan metabolism},

  author       = {Mayer, D and Branscheid, D},

  abstractNote = {Exposure of cultures of human lung fibroblasts to 0.5 ppm ozone for 20 h resulted in a significant increase in cellular mortality by 29%; after exposure to 2.5 ppm ozone for 4 h, the increase amounted to 74%. A marked difference in sensitivity to ozone was observed between fibroblast lines from different individuals. This variability in resistance to ozone was more evident after exposure to 0.5 ppm ozone for 20 h, when compared with 2.5 ppm ozone for 4 h. In one fibroblast line, synthesis of hyaluronan was enhanced by exposure to 0.5 ppm ozone for 20 h. The concentrations of hyaluronan in culture media increased in experiments using different fibroblast cell lines, a phenomenon that was obvious both if cell numbers and combined protein concentrations of cells and media are selected as references for hyaluronan concentrations.},

  doi          = {10.1080/15287399209531614},

  url          = {https://www.osti.gov/biblio/5227045},
  journal      = {Journal of Toxicology and Environmental Health; (United States)},

  issn         = {0098-4108},

  number       = ,

  volume       = 35:4,

  place        = {United States},

  year         = {Wed Apr 01 00:00:00 EST 1992},

  month        = {Wed Apr 01 00:00:00 EST 1992}

}

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