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Title: Stability of alginate-immobilized algal cells

Abstract

Investigations were carried out using immobilized Chlorella cells to determine the diameter, compressibility, tolerance to phosphate chelation, and ability to retain algal cells during incubation of various alginate beads. These physical bead-characteristics were affected by a variety of interactive factors, including multivalent cation type (hardening agent) and cell, cation, and alginate concentration, the latter exhibiting a predominant influence. The susceptibility of alginate beads to phosphate chelation involved a complex interaction of cation type, concentration, and pH of phosphate solution. A scale of response ranging from gel swelling to gel shrinking was observed for a range of conditions. However, stable Ca alginate beads were maintained in incubation media with a pH of 5.5 and a phosphate concentration of 5 micro M. A preliminary investigation into cell leakage from the beads illustrated the importance of maintaining a stable gel structure and limiting cell growth to reduce leakage.

Authors:
; ; ; ;
Publication Date:
Research Org.:
School of Natural Science, Hatfield/Herts, England
OSTI Identifier:
5218521
Resource Type:
Journal Article
Journal Name:
Biotechnol. Bioeng.; (United States)
Additional Journal Information:
Journal Volume: 28:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS; CHLORELLA; IMMOBILIZED CELLS; STABILITY; ALGAE; ALGINATES; CATIONS; CHELATING AGENTS; COMPRESSIBILITY; GELS; GROWTH; INCUBATION; LEAKS; PH VALUE; PHOSPHATES; PLANT CELLS; CHARGED PARTICLES; COLLOIDS; DISPERSIONS; IONS; MECHANICAL PROPERTIES; MICROORGANISMS; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PLANTS; UNICELLULAR ALGAE; 550700* - Microbiology; 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)

Citation Formats

Dainty, A L, Goulding, K H, Robinson, P K, Simpkins, I, and Trevan, M D. Stability of alginate-immobilized algal cells. United States: N. p., 1986. Web. doi:10.1002/bit.260280210.
Dainty, A L, Goulding, K H, Robinson, P K, Simpkins, I, & Trevan, M D. Stability of alginate-immobilized algal cells. United States. https://doi.org/10.1002/bit.260280210
Dainty, A L, Goulding, K H, Robinson, P K, Simpkins, I, and Trevan, M D. 1986. "Stability of alginate-immobilized algal cells". United States. https://doi.org/10.1002/bit.260280210.
@article{osti_5218521,
title = {Stability of alginate-immobilized algal cells},
author = {Dainty, A L and Goulding, K H and Robinson, P K and Simpkins, I and Trevan, M D},
abstractNote = {Investigations were carried out using immobilized Chlorella cells to determine the diameter, compressibility, tolerance to phosphate chelation, and ability to retain algal cells during incubation of various alginate beads. These physical bead-characteristics were affected by a variety of interactive factors, including multivalent cation type (hardening agent) and cell, cation, and alginate concentration, the latter exhibiting a predominant influence. The susceptibility of alginate beads to phosphate chelation involved a complex interaction of cation type, concentration, and pH of phosphate solution. A scale of response ranging from gel swelling to gel shrinking was observed for a range of conditions. However, stable Ca alginate beads were maintained in incubation media with a pH of 5.5 and a phosphate concentration of 5 micro M. A preliminary investigation into cell leakage from the beads illustrated the importance of maintaining a stable gel structure and limiting cell growth to reduce leakage.},
doi = {10.1002/bit.260280210},
url = {https://www.osti.gov/biblio/5218521}, journal = {Biotechnol. Bioeng.; (United States)},
number = ,
volume = 28:2,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1986},
month = {Wed Jan 01 00:00:00 EST 1986}
}