Persistent stimulation of adenylate cyclase and urea transport by an AVP photolabel

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Am. J. Physiol.; (United States)

OSTI ID:5217867

Eggena, P; Ma, C L; Fahrenholz, F; Schwartz, I L

The effects of a photoaffinity label for arginine vasopressin receptors, (Phe2, Phe(p-N3)3)AVP (N3-AVP), on urea permeability and adenylate cyclase activity have been investigated in the toad urinary bladder. This compound, when activated by ultraviolet light, induced a maximal and persistent increase in the urea permeability of the intact bladder and a persistent increase in the adenylate cyclase activity of toad bladder epithelial cell homogenates. Covalent attachment of the analogue to target tissue during photolysis was equivalent at 4 and 20 degrees C. Bladders exposed to N3-AVP in the presence of AVP during photolysis were substantially less permeable to urea than controls that had been exposed to N3-AVP alone. These findings constitute further evidence in support of the previous suggestion that N3-AVP binds covalently to AVP receptors and, in addition, demonstrates that N3-AVP evokes a persistent increase in adenylate cyclase activity which, in turn, triggers a persistent increase in bladder permeability to urea.

• Research Organization: Mount Sinai Medical, New York, NY
• OSTI ID: 5217867
• Journal Information: Am. J. Physiol.; (United States), Journal Name: Am. J. Physiol.; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BLADDER
PERMEABILITY
CYCLASES
ENZYME ACTIVITY
UREA
MEMBRANE TRANSPORT
VASOPRESSIN
BIOLOGICAL RADIATION EFFECTS
RECEPTORS
AFFINITY
ARGININE
CHEMICAL BONDS
COVALENCE
EPITHELIUM
FROGS
HOMOGENATES
IN VITRO
PHOTOCHEMISTRY
PHOTOLYSIS
ULTRAVIOLET RADIATION
AMIDES
AMINO ACIDS
AMPHIBIANS
ANIMAL TISSUES
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BODY
CARBONIC ACID DERIVATIVES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ENZYMES
HORMONES
LYASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOTOCHEMICAL REACTIONS
PITUITARY HORMONES
RADIATION EFFECTS
RADIATIONS
TISSUES
URINARY TRACT
VERTEBRATES
560111* - Radiation Effects on Biochemicals- In Vitro- (-1987)