In vitro induction of sister chromatid exchanges and chromosomal aberrations in peripheral lymphocytes of the oyster toadfish and American eel
A series of experiments was conducted to characterize the proliferation of oyster toadfish lymphocytes in medium containing 5-bromodeoxyuridine (BrdUrd) and to determine the effectiveness of cytogenetic endpoints for assessing the genotoxic effects of in vitro exposure of toadfish and eel lymphocytes to known mammalian clastogens. Although the rate of proliferation of toadfish lymphocytes was low compared to that of mammalian lymphocytes, the effects of increasing BrdUrd concentrations were similar. Mitomycin C (MMC) and ethylene dibromide (EDB) induced concentration-dependent increases in chromatid-type exchange and SCE frequencies with least effective concentrations for SCE induction by MMC (6.8 x 10/sup -9/ M) and EDB (2.6 x 10/sup -4/ M) that were comparable to or slightly lower than those that have been obtained with mammalian in vitro systems. In vitro exposure of toadfish lymphocytes to dimethoate (DIM) induced a concentration-dependent increase in SCE frequency with a least effective concentration of 2.8 x 10/sup -3/ M that was much higher than that observed with mammalian in vitro systems. In vitro exposure of American eel lymphocytes to MMC also induced a concentration-dependent increase in the frequency of chromosomal aberrations and SCEs with a least effective concentration for SCE induction of 2.0 x 10/sup -9/ M. These results indicate that cytogenetic endpoints can be effectively scored with cultured lymphocytes from these and perhaps other fish species with comparable karyotypes that contain an average of at least 0.07 pg DNA/chromosome.
- Research Organization:
- Univ. of North Carolina at Chapel Hill, Morehead City
- OSTI ID:
- 5217461
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 8:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BROMIDES
BIOLOGICAL EFFECTS
CHROMOSOMAL ABERRATIONS
MUTATION FREQUENCY
MITOMYCIN
SISTER CHROMATID EXCHANGES
BUDR
CELL PROLIFERATION
EEL
FISHES
LYMPHOCYTES
WATER POLLUTION
ANIMAL CELLS
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMETABOLITES
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AQUATIC ORGANISMS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
BROMINE COMPOUNDS
BROMOURACILS
CONNECTIVE TISSUE CELLS
DRUGS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
LEUKOCYTES
MATERIALS
MUTATIONS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
POLLUTION
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
URACILS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)