Free radicals promote in vitro intracellular decay of rabbit reticulocyte and erythrocyte hexokinase

Conference · Fri Mar 15 00:00:00 EST 1991 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:5213604

Stocchi, V; Biagiarelli, B; Masat, L; Palma, F; Piccoli, G; Cucchiarini, L ^[1]

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1. Urbino Univ. (Italy)

The authors studied the behavior of enzymes involved in the glycolytic pathway incubating intact reticulocyte and erythrocyte at 37C in the presence of ascorbic acid and Fe{sup 2+}. The results obtained have shown evidence that among the glycolytic enzymes the hexokinase activity shows a pronounced decay. For this reason the authors have investigated how the chromatographic profile of hexokinase changes after exposure of reticulocytes and erythrocytes to the oxygen-radical generating system in trying to understand the molecular basis of this inactivation. The results obtained have shown a different effect of free radicals on the reticulocyte and erythrocyte hexokinase molecular forms. The analysis of the chromatographic profile was performed using a TSK Gel Toyopearl DEAE 650 S column which allows a complete resolution of the distinct forms of hexokinase together with a complete recovery of the enzyme activities. Concomitantly to the hexokinase decay, there is a fall in the GSH level when intact rabbit erythrocytes and reticulocytes are incubated in presence of iron and ascorbic acid. However, the fall of GSH is significantly higher in the erythrocytes where, after incubation of one hour, it reaches a mean value of 0.3 {mu}moles per ml of cells representing about 10% of the initial value. In the reticulocytes the GSH value, after the same treatment, remain as high as 1.6 {mu}moles per ml of cells. If we consider that the initial value of GSH is almost the same in the erythrocytes are reticulocytes the highest decay rate of hexokinase observed in these latter cells, cannot be related to the fall of the GSH level, but to a possible direct effect of free-radicals on the enzyme.

• OSTI ID: 5213604
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:5; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GLUTATHIONE
BIOSYNTHESIS
HEXOKINASE
ENZYME ACTIVITY
RADICALS
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CHROMATOGRAPHY
ERYTHROCYTES
IN VITRO
RABBITS
RETICULOCYTES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DRUGS
ENZYMES
MAMMALS
MATERIALS
ORGANIC COMPOUNDS
PEPTIDES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHOTRANSFERASES
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
SEPARATION PROCESSES
SYNTHESIS
TRANSFERASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology