Vaccinia virus, herpes simplex virus, and carcinogens induce DNA amplification in a human cell line and support replication of a helpervirus dependent parvovirus
The SV40-transformed human kidney cell line, NB-E, amplifies integrated as well as episomal SV40 DNA upon treatment with chemical (DMBA) or physical (uv irradiation) carcinogens (initiators) as well as after infection with herpes simplex virus (HSV) type 1 or with vaccinia virus. In addition it is shown that vaccinia virus induces SV40 DNA amplification also in the SV40-transformed Chinese hamster embryo cell line, CO631. These findings demonstrate that human cells similar to Chinese hamster cells amplify integrated DNA sequences after treatment with carcinogens or infection with specific viruses. Furthermore, a poxvirus--vaccinia virus--similar to herpes group viruses induces DNA amplification. As reported for other systems, the vaccinia virus-induced DNA amplification in NB-E cells is inhibited by coinfection with adeno-associated virus (AAV) type 5. This is in line with previous studies on inhibition of carcinogen- or HSV-induced DNA amplification in CO631 cells. The experiments also demonstrate that vaccinia virus, in addition to herpes and adenoviruses acts as a helper virus for replication and structural antigen synthesis of AAV-5 in NB-E cells.
- Research Organization:
- Institut fuer Virusforschung, Heidelberg, Germany, F.R.
- OSTI ID:
- 5204173
- Journal Information:
- Virology; (United States), Vol. 1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DIMETHYLBENZANTHRACENE
GENETIC EFFECTS
DNA
GENE AMPLIFICATION
ULTRAVIOLET RADIATION
VACCINIA VIRUS
ANIMAL CELLS
CARCINOGENS
CELL CULTURES
CHO CELLS
DNA REPLICATION
KIDNEYS
AROMATICS
BIOLOGICAL EFFECTS
BODY
CONDENSED AROMATICS
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PARASITES
RADIATIONS
VIRUSES
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