The human CYP2F gene subfamily: Identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping
- National Institutes of Health, Bethesda, MD (USA)
A cDNA coding for a P450, designated IIF1, was isolated from a human lung {lambda}gt11 library by screening with a human IIC9 cDNA probe. The cDNA-encoded IIF1 protein had 491 amino acids and a calculated molecular weight of 55,507. IIF1 cDNA, expressed by using a vaccinia virus vector, produced a cytochrome with a {lambda}{sub max} of 454 nm when reduced and complexed with carbon monoxide. This enzyme was able to dealkylate ethoxycoumarin, propoxycoumarin, and pentoxyresorufin but possessed no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin. A variant cDNA, designated IIF1v, was isolated that was identical with IIF1 except for the loss of two segments of 161 and 388 bp within the cDNA coding region. Two mRNAs, consistent with the predicted size of the IIF1 and IIF1v transcripts, were found at very low abundance in lung specimens by Northern blot analysis. A 2-kb transcript, hybridizing with the human IIF1, was also detected as an abundant mRNA in rat lung. The CYP2F gene subfamily was localized to human chromosome 19 and mouse chromosome 7. On the basis of southern blotting analysis with multiple restriction enzymes, the authors conclude that the CYP2F1 gene is flanked by a second highly similar gene.
- OSTI ID:
- 5200863
- Journal Information:
- Biochemistry; (United States), Vol. 29:23; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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CYTOCHROMES
DNA SEQUENCING
GENETIC MAPPING
HUMAN CHROMOSOME 19
AMINO ACID SEQUENCE
DNA HYBRIDIZATION
LUNGS
MAN
RATS
RECOMBINANT DNA
ANIMALS
BODY
CHROMOSOMES
DNA
HYBRIDIZATION
MAMMALS
MAPPING
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PIGMENTS
PRIMATES
PROTEINS
RESPIRATORY SYSTEM
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
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550201* - Biochemistry- Tracer Techniques
550401 - Genetics- Tracer Techniques