Effect of dexamethasone on the cytotoxic and enzymatic response of cultured endothelial cells to radiation
Experiments were conducted to determine (1) whether glucocorticoids directly protected endothelial cells (EC) from radiation and (2) if angiotensin converting enzyme (ACE) activity, known to be increased by glucocorticoid, played a role in the EC response to radiation. Confluent monolayers of EC cultured from bovine aorta EC were treated with dexamethasone (10/sup -6/ M); after irradiation (5.0 Gy, /sup 60/Co ..gamma..) ACE and lactate dehydrogenase (LDH) activities, DNA and protein contents, and nuclei number were measured. Combined dexamethasone treatment and radiation increased cellular ACE activity at a time when neither agent alone had an effect (24-hr dexamethasone exposure before 5 Gy and assayed 24 hr after 5 Gy). This interaction between radiation and dexamethasone treatment suggests that the glucocorticoid modifies the cell's response to injury. Although this interaction does not ameliorate radiation cytotoxicity, maintenance of ACE levels in injured vessels by hormones may have physiological significance in the hemodynamics of irradiated tissues.
- Research Organization:
- Northwestern Univ. Medical School, Chicago, IL
- OSTI ID:
- 5198444
- Journal Information:
- Radiat. Res.; (United States), Vol. 103:2
- Country of Publication:
- United States
- Language:
- English
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DEXAMETHASONE
RADIOSENSITIVITY EFFECTS
ENDOTHELIUM
BIOLOGICAL RADIATION EFFECTS
ANGIOTENSIN
ANIMAL CELLS
AORTA
CELL NUCLEI
COBALT 60
DNA
ENZYME ACTIVITY
GAMMA RADIATION
LACTATE DEHYDROGENASE
PROTEINS
TOXICITY
ADRENAL HORMONES
ANIMAL TISSUES
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
COBALT ISOTOPES
CORTICOSTEROIDS
DRUGS
ELECTROMAGNETIC RADIATION
ENZYMES
GLOBULINS
GLUCOCORTICOIDS
HEMIACETAL DEHYDROGENASES
HORMONES
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KETONES
MINUTES LIVING RADIOISOTOPES
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
PREGNANES
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
STEROID HORMONES
STEROIDS
TISSUES
VASOCONSTRICTORS
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)