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Title: Cytoplasmic membrane is the target organelle for transition metal mediated damage induced by paraquat in Escherichia coli

Abstract

Bacterial survival indicates that copper or iron is an essential mediator in paraquat toxicity in Escherichia coli. In this study the authors have identified the cytoplasmic membrane as a target organelle in metal-mediated paraquat toxicity and have demonstrated the complete correlation of the membrane damage with the levels of adventitious copper (or iron). The extent of membrane damage was related by use of four parameters: (a) the level of cellular ATP, (b) the level of cellular potassium, (c) the cellular capacity to accumulate and retain radiolabeled leucine, and (d) the cellular integrity as reflected by transmission electron microscopy (TEM). Exposure of bacterial cells to a combination of paraquat and copper caused a marked decline in parameters a, b, and c. This decline was found to occur in parallel with, or even to precede, the sharp loss of survival of E. coli under the same conditions. Likewise, TEM micrographs clearly indicated alternations in cellular structure that possibly reflect sites of detachment of the cytoplasmic membrane from the bacterial capsule. In contradistinction, copper alone or paraquat alone could not bring about similar changes in cellular structure. These findings are in accord with the suggested site-specific metal-mediated Haber-Weiss mechanism for paraquat toxicity andmore » support our notion that specific chelators of transition metals could reduce or prevent the biological deleterious effects of this herbicide.« less

Authors:
;
Publication Date:
Research Org.:
Hebrew Univ. of Jerusalem (Israel)
OSTI Identifier:
5195041
Resource Type:
Journal Article
Journal Name:
Biochemistry; (United States)
Additional Journal Information:
Journal Volume: 27:7
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BIPYRIDINES; TOXICITY; CARBON 14 COMPOUNDS; UPTAKE; METALS; ATP; CATALASE; CATIONS; CELL MEMBRANES; COPPER COMPOUNDS; ESCHERICHIA COLI; HERBICIDES; INACTIVATION; IRON COMPOUNDS; LEUCINE; POTASSIUM COMPOUNDS; TRANSMISSION ELECTRON MICROSCOPY; ALKALI METAL COMPOUNDS; AMINO ACIDS; AZINES; BACTERIA; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CHARGED PARTICLES; ELECTRON MICROSCOPY; ELEMENTS; ENZYMES; HETEROCYCLIC COMPOUNDS; IONS; LABELLED COMPOUNDS; MEMBRANES; MICROORGANISMS; MICROSCOPY; NUCLEOTIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDOREDUCTASES; PEROXIDASES; PESTICIDES; PYRIDINES; TRANSITION ELEMENT COMPOUNDS; 560300* - Chemicals Metabolism & Toxicology; 550701 - Microbiology- Tracer Techniques

Citation Formats

Kohen, R, and Chevion, M. Cytoplasmic membrane is the target organelle for transition metal mediated damage induced by paraquat in Escherichia coli. United States: N. p., 1988. Web. doi:10.1021/bi00407a049.
Kohen, R, & Chevion, M. Cytoplasmic membrane is the target organelle for transition metal mediated damage induced by paraquat in Escherichia coli. United States. https://doi.org/10.1021/bi00407a049
Kohen, R, and Chevion, M. 1988. "Cytoplasmic membrane is the target organelle for transition metal mediated damage induced by paraquat in Escherichia coli". United States. https://doi.org/10.1021/bi00407a049.
@article{osti_5195041,
title = {Cytoplasmic membrane is the target organelle for transition metal mediated damage induced by paraquat in Escherichia coli},
author = {Kohen, R and Chevion, M},
abstractNote = {Bacterial survival indicates that copper or iron is an essential mediator in paraquat toxicity in Escherichia coli. In this study the authors have identified the cytoplasmic membrane as a target organelle in metal-mediated paraquat toxicity and have demonstrated the complete correlation of the membrane damage with the levels of adventitious copper (or iron). The extent of membrane damage was related by use of four parameters: (a) the level of cellular ATP, (b) the level of cellular potassium, (c) the cellular capacity to accumulate and retain radiolabeled leucine, and (d) the cellular integrity as reflected by transmission electron microscopy (TEM). Exposure of bacterial cells to a combination of paraquat and copper caused a marked decline in parameters a, b, and c. This decline was found to occur in parallel with, or even to precede, the sharp loss of survival of E. coli under the same conditions. Likewise, TEM micrographs clearly indicated alternations in cellular structure that possibly reflect sites of detachment of the cytoplasmic membrane from the bacterial capsule. In contradistinction, copper alone or paraquat alone could not bring about similar changes in cellular structure. These findings are in accord with the suggested site-specific metal-mediated Haber-Weiss mechanism for paraquat toxicity and support our notion that specific chelators of transition metals could reduce or prevent the biological deleterious effects of this herbicide.},
doi = {10.1021/bi00407a049},
url = {https://www.osti.gov/biblio/5195041}, journal = {Biochemistry; (United States)},
number = ,
volume = 27:7,
place = {United States},
year = {Tue Apr 05 00:00:00 EDT 1988},
month = {Tue Apr 05 00:00:00 EDT 1988}
}