Allosteric and catalytic binding of S-adenosylmethionine to Escherichia coli DNA adenine methyltransferase monitored by 3H NMR
Journal Article
·
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
- Departement de Biologie Cellulaire et Moleculaire, Centre d'Etudes de Saclay, Gif-sur-Yvette (France)
Adenine methylation of GATC sequences in DNA is carried out by the DNA adenine methyltransferase with the methyl group source being the cofactor S-adenosylmethionine. The authors report 3H NMR studies on the interaction of DNA adenine methyltransferase with S-adenosylmethionine and the reaction when the ternary complex is formed with an oligonucleotide containing a GATC site. The methylation reaction was also studied in the presence of a competitive inhibitor and this showed two successive stages involved in the methylation and two sites of binding for S-adenosylmethionine.
- OSTI ID:
- 5191226
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:15; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ADENINES
METHYLATION
METHIONINE
BIOCHEMICAL REACTION KINETICS
METHYL TRANSFERASES
ENZYME ACTIVITY
DNA
DNA SEQUENCING
ENZYME INHIBITORS
ESCHERICHIA COLI
NUCLEAR MAGNETIC RESONANCE
OLIGONUCLEOTIDES
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
BACTERIA
CARBON-GROUP TRANSFERASES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
PURINES
REACTION KINETICS
RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques
ADENINES
METHYLATION
METHIONINE
BIOCHEMICAL REACTION KINETICS
METHYL TRANSFERASES
ENZYME ACTIVITY
DNA
DNA SEQUENCING
ENZYME INHIBITORS
ESCHERICHIA COLI
NUCLEAR MAGNETIC RESONANCE
OLIGONUCLEOTIDES
RECEPTORS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANTIMETABOLITES
AROMATICS
AZAARENES
BACTERIA
CARBON-GROUP TRANSFERASES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DRUGS
ENZYMES
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPOTROPIC FACTORS
MAGNETIC RESONANCE
MEMBRANE PROTEINS
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
PURINES
REACTION KINETICS
RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
550201* - Biochemistry- Tracer Techniques