Regulation of the human. beta. -actin promoter by upstream and intron domains

Journal Article · Wed Jan 25 00:00:00 EST 1989 · Nucleic Acids Research; (UK)

DOI:https://doi.org/10.1093/nar/17.2.601· OSTI ID:5190876

Ng, Sunyu ^[1]; Gunning, P; Kedes, L ^[2]; Liu, Shuhui ^[3]; Leavitt, J ^[4]

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1. Linus Pauling Institute of Science and Medicine, Palo Alto, CA (USA); Institute of Molecular Biology, Nankang (China)
2. Stanford Univ. School of Medicine, Palo Alto, CA (USA)
3. Institute of Molecular Biology, Taipei (China) National Tsing Hua Univ., Hsinchu (China)
4. Linus Pauling Institute of Science and Medicine, Palo Alto, CA (USA)

The authors have identified three regulatory domains of the complex human {beta}-actin gene promoter. They span a region of about 3,000 bases, from not more than {minus}2,011 bases upstream of the mRNA cap site to within the 5{prime} intron (832 bases long). A distal upstream domain contains at least one enhancer-like element. A proximal upstream domain, with a CArG (for CC(A+T rich){sub 6}GG) motif found in all known mammalian actin genes, seems to confer serum, but not growth factor, inducibility. The third domain is within the evolutionarily conserved 3{prime} region of the first intron and contains a 13 base-pair sequence, identical to the upstream sequence with the CArG motif. This domain also contains sequences that are both serum and fibroblast growth inducible.

• OSTI ID: 5190876
• Journal Information: Nucleic Acids Research; (UK), Vol. 17:2; ISSN 0305-1048
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REPRESSORS
GENE REGULATION
GENES
CHEMICAL ACTIVATION
PLASMIDS
DNA SEQUENCING
ACTIN
BLOOD SERUM
CODONS
FIBROBLASTS
GROWTH FACTORS
MAN
ANIMAL CELLS
ANIMALS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
MAMMALS
MITOGENS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
SOMATIC CELLS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry