Biochemical and biological properties of the nerve growth factor receptor
Abstract
We have utilized a monoclonal antibody (192-IgG) to study the rat nerve growth factor receptor. After intraocular injection, {sup 125}I-192-IgG was retrogradely transported in sympathetic neuronal axons to the superior cervical ganglion. When the sciatic nerve was ligated to induce the accumulation of axonally transported materials, 192-IgG immunostaining was observed on both sides of the ligature, indicating that NGF receptors are transported in both orthograde and retrograde directions. By using {sup 125}I-NGF crosslinking and 192-IgG immunoprecipitation, we detected receptor molecules throughout the rat brain, thereby supporting the hypothesis that NGF is active in the central nervous system. We also discovered that sciatic nerve transection leads to a dramatic increase in the amount of NGF receptor found in the distal portion of the nerve. Immunostaining revealed that all Schwann cells in the distal axotomized nerve were expressing NGF receptors. We examined phosphorylation of NGF receptor in cultured sympathetic neurons and PC12 cells. We also examined pharmacological effects of 192-IgG. Systemic injection of 192-IgG into neonatal rats caused a permanent partial sympathectomy in a dose-dependent manner; a maximum of 50% of the cells were killed.
- Authors:
- Publication Date:
- Research Org.:
- Washington Univ., Seattle, WA (USA)
- OSTI Identifier:
- 5188073
- Resource Type:
- Thesis/Dissertation
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; RECEPTORS; CHEMICAL PROPERTIES; BIOCHEMICAL REACTION KINETICS; BRAIN; CROSS-LINKING; DOSE-RESPONSE RELATIONSHIPS; IMMUNOASSAY; IODINE 125; MONOCLONAL ANTIBODIES; NERVES; PHOSPHORYLATION; RATS; TRACER TECHNIQUES; ANIMALS; ANTIBODIES; BETA DECAY RADIOISOTOPES; BIOASSAY; BODY; CENTRAL NERVOUS SYSTEM; CHEMICAL REACTIONS; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; POLYMERIZATION; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
Citation Formats
Taniuchi, M. Biochemical and biological properties of the nerve growth factor receptor. United States: N. p., 1988.
Web.
Taniuchi, M. Biochemical and biological properties of the nerve growth factor receptor. United States.
Taniuchi, M. 1988.
"Biochemical and biological properties of the nerve growth factor receptor". United States.
@article{osti_5188073,
title = {Biochemical and biological properties of the nerve growth factor receptor},
author = {Taniuchi, M},
abstractNote = {We have utilized a monoclonal antibody (192-IgG) to study the rat nerve growth factor receptor. After intraocular injection, {sup 125}I-192-IgG was retrogradely transported in sympathetic neuronal axons to the superior cervical ganglion. When the sciatic nerve was ligated to induce the accumulation of axonally transported materials, 192-IgG immunostaining was observed on both sides of the ligature, indicating that NGF receptors are transported in both orthograde and retrograde directions. By using {sup 125}I-NGF crosslinking and 192-IgG immunoprecipitation, we detected receptor molecules throughout the rat brain, thereby supporting the hypothesis that NGF is active in the central nervous system. We also discovered that sciatic nerve transection leads to a dramatic increase in the amount of NGF receptor found in the distal portion of the nerve. Immunostaining revealed that all Schwann cells in the distal axotomized nerve were expressing NGF receptors. We examined phosphorylation of NGF receptor in cultured sympathetic neurons and PC12 cells. We also examined pharmacological effects of 192-IgG. Systemic injection of 192-IgG into neonatal rats caused a permanent partial sympathectomy in a dose-dependent manner; a maximum of 50% of the cells were killed.},
doi = {},
url = {https://www.osti.gov/biblio/5188073},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}