Effects of different enzyme treatments in extraction of total folate from infant formula, baby foods and other food products prior to microbiological assay and radioassay

Thesis/Dissertation · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5187986

De Souza, S C

Four different enzyme treatments-conjugase alone, conjugase and alpha-amylase, conjugase and Pronase{reg sign} and a triple enzyme combination of conjugase, Pronase {reg sign} and alpha-amylase were applied in the extraction of total folate from infant formula, baby foods and various other foods by microbiological and radioassay methods. Significant increases (P < 0.05) in measurable folate were obtained using the triple enzyme system in spinach, Camembert cheese, soy-based infant formula and cereal-based, meat-based and fruit-based infant foods over the use of conjugase alone by the microbiological method. Increases were also observed in many of the same foods using Pronase{reg sign} or alpha-amylase in addition to conjugase alone. Increases obtained by microbiological assay were confirmed by radioassay in a number of foods studied.

• Research Organization: Georgia Inst. of Tech., Atlanta, GA (USA)
• OSTI ID: 5187986
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FOLIC ACID
EXTRACTION
FOOD
RADIOASSAY
MILK
AMYLASE
INFANTS
MICROORGANISMS
TRYPSIN
AGE GROUPS
AMINO ACIDS
AROMATICS
AZAARENES
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
CHILDREN
DRUGS
ENZYMES
GLYCOSYL HYDROLASES
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY COMPOUNDS
MATERIALS
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDE HYDROLASES
PTERIDINES
SEPARATION PROCESSES
SERINE PROTEINASES
VITAMIN B GROUP
VITAMINS
550201* - Biochemistry- Tracer Techniques