An intron capture strategy used to identify and map a lysyl oxidase-like gene on chromosome 9 in the mouse
- Rutgers Univ., Piscataway, NJ (United States)
- UMDNJ, New Brunswick, NJ (United States)
An intron capture strategy involving use of polymerase chain reaction was used to identify and map the mouse homologue of a human lysyl oxidase-like gene (LOXL). Oligonucleotides complementary to conserved domains within exons 4 and 5 of the human lysyl oxidase-like gene were used to amplify the corresponding segment from mouse genomic DNA. Sequencing of the resulting mouse DNA fragment of approximately 1 kb revealed that the exon sequences at the ends of the amplified fragment are highly homologous (90% nucleotide identity) to exons 4 and 5 of the human lysyl oxidase-like gene. An AluI restriction site polymorphism within intron 4 was used to map the mouse lysyl oxidase-like gene (Loxl) to mouse Chromosome 9 in a region that shares linkage conservation with human chromosome 15q24, to which the LOXL was recently mapped. 22 refs., 3 figs.
- OSTI ID:
- 518482
- Journal Information:
- Genomics, Vol. 40, Issue 2; Other Information: PBD: 1 Mar 1997
- Country of Publication:
- United States
- Language:
- English
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