Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries

Title: Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries

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Abstract

Previous in vitro studies with the calcium channel blockers (CCB) indirectly suggest that K/sup +/ and serotonin (5HT) constrict bovine middle cerebral arteries (BMCA) by promoting the influx of extracellular calcium (Ca) through CCB-sensitive channels. In this study, the authors directly determined the sources of activator Ca for K/sup +/- and 5HT-induced constriction of BMCA, using radiolabelled /sup 4/)2%Ca and /sup 3/H-sorbitol. EGTA-resistant Ca uptake, an estimate of Ca influx into vascular smooth muscle, was determined by exposure to Ca-deficient 2 mM EGTA solutions at 1/sup 0/C. The total Ca content of BMCA was 4.4 nmole/mg (wet wt.) after equilibration at 37/sup 0/C. The total exchangeable Ca content was 1.64 nmole/mg after 1 hr of /sup 45/Ca loading; the Ca content of the extracellular water was 0.30 nmole/mg, as estimated from the /sup 3/H-sorbitol space (0.25 ul/mg). The EGTA-resistant Ca uptake at 1 hr was 134 pmole/mg. K/sup +/ and 5HT significantly increased EGTA-resistant Ca uptake during 5 min of /sup 45/Ca loading; for K/sup +/, Ca uptake increased from 71 to 202 pmole/mg, and for 5HT, from 65 to 102 pmole/mg. Verapamil (10/sup -5/ M) or nifedipine (3.3 x 10/sup -7/ M) significantly blocked the increase in EGTA-resistant Camore »« less

Publication Date:: Sat Mar 01 00:00:00 EST 1986

Research Org.:: Temple Univ. School of Medicine, Philadelphia, PA

OSTI Identifier:: 5179235

Report Number(s):: CONF-8604222-
Journal ID: CODEN: FEPRA

Resource Type:: Conference

Journal Name:: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

Additional Journal Information:: Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ARTERIES; VASOCONSTRICTION; CALCIUM; MEMBRANE TRANSPORT; POTASSIUM; BIOLOGICAL EFFECTS; SEROTONIN; CALCIUM 45; CATTLE; MUSCLES; SORBITOL; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ALKALI METALS; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; AMINES; ANIMALS; AROMATICS; AUTONOMIC NERVOUS SYSTEM AGENTS; AZAARENES; AZOLES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BLOOD VESSELS; BODY; CALCIUM ISOTOPES; CARBOHYDRATES; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DIURETICS; DOMESTIC ANIMALS; DRUGS; ELEMENTS; EVEN-ODD NUCLEI; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LABELLED COMPOUNDS; MAMMALS; METALS; MONOSACCHARIDES; NEUROREGULATORS; NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PYRROLES; RADIOISOTOPES; RADIOPROTECTIVE SUBSTANCES; RUMINANTS; SACCHARIDES; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

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                    . Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries.  United States: N. p., 1986. 
        Web.

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                    . Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries.  United States.

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                    . 1986.  
        "Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries".  United States.

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@article{osti_5179235,

  title        = {Sources of activator calcium for potassium- and serotonin-induced constriction of isolated bovine cerebral arteries},

  author       = {},

  abstractNote = {Previous in vitro studies with the calcium channel blockers (CCB) indirectly suggest that K/sup +/ and serotonin (5HT) constrict bovine middle cerebral arteries (BMCA) by promoting the influx of extracellular calcium (Ca) through CCB-sensitive channels. In this study, the authors directly determined the sources of activator Ca for K/sup +/- and 5HT-induced constriction of BMCA, using radiolabelled /sup 4/)2%Ca and /sup 3/H-sorbitol. EGTA-resistant Ca uptake, an estimate of Ca influx into vascular smooth muscle, was determined by exposure to Ca-deficient 2 mM EGTA solutions at 1/sup 0/C. The total Ca content of BMCA was 4.4 nmole/mg (wet wt.) after equilibration at 37/sup 0/C. The total exchangeable Ca content was 1.64 nmole/mg after 1 hr of /sup 45/Ca loading; the Ca content of the extracellular water was 0.30 nmole/mg, as estimated from the /sup 3/H-sorbitol space (0.25 ul/mg). The EGTA-resistant Ca uptake at 1 hr was 134 pmole/mg. K/sup +/ and 5HT significantly increased EGTA-resistant Ca uptake during 5 min of /sup 45/Ca loading; for K/sup +/, Ca uptake increased from 71 to 202 pmole/mg, and for 5HT, from 65 to 102 pmole/mg. Verapamil (10/sup -5/ M) or nifedipine (3.3 x 10/sup -7/ M) significantly blocked the increase in EGTA-resistant Ca uptake induced by K/sup +/ or 5HT. These results provide direct evidence that K/sup +/ or 5HT may constrict BMCA by promoting the influx of extracellular Ca through CCB-sensitive channels.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5179235},
  journal      = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number       = ,

  volume       = 45:3,

  place        = {United States},

  year         = {Sat Mar 01 00:00:00 EST 1986},

  month        = {Sat Mar 01 00:00:00 EST 1986}

}

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