Relationship of hormone-sensitive and hormone-insensitive phosphatidylinositol to phosphatidylinositol 4,5-bisphosphate in the WRK-1 cell

Journal Article · Sun Jan 05 00:00:00 EST 1986 · J. Biol. Chem.; (United States)

OSTI ID:5179213

Two distinct pools of phosphatidylinositol (PI) in the WRK-1 rat mammary tumor cell, one whose metabolism is enhanced in response to vasopressin and another which is insensitive to hormonal manipulation have been previously characterized. The purpose of the present study was to examine the relationship between cellular phosphatidylinositol 4,5-bisphosphate (PIP/sub 2/) and each of the two PI pools. In WRK-1 cells, vasopressin induces the rapid loss of PIP/sub 2/ and the accumulation of inositol phosphates. By making use of kinetic differences in /sup 32/P/sub i/ uptake into the two pools of PI and assessing radioactivity levels in the 1-phosphate of PIP/sub 2/, it was determined that hormone-sensitive PI is the precursor of approximately 60% of the cellular PIP/sub 2/; the remainder is synthesized from the hormone-insensitive pool. Additional data indicate that PIP/sub 2/ derived from hormone-sensitive PI is likewise hormone-sensitive, while that synthesized from hormone-insensitive PI remains stable over a long period of time and is not affected by the presence of vasopressin.

• Research Organization: New York Univ. Medical Center
• OSTI ID: 5179213
• Journal Information: J. Biol. Chem.; (United States), Vol. 261:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INOSITOLS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
TUMOR CELLS
METABOLISM
VASOPRESSIN
EXPERIMENTAL DATA
LABELLED COMPOUNDS
MAMMARY GLANDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHATES
PHOSPHORUS 32
PRECURSOR
RATS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOHYDRATES
DATA
DAYS LIVING RADIOISOTOPES
GLANDS
HORMONES
INFORMATION
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PITUITARY HORMONES
RADIOISOTOPES
REACTION KINETICS
RODENTS
SACCHARIDES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques