Cyclic nucleotide-dependent phosphorylation of proteins in rod outer segments in frog retina: characteristics of the phosphorylated proteins and their dephosphorylation
To clarify the function of cyclic nucleotides in rod outer segments (ROS) of frog retinas, the cyclic nucleotide-dependent phosphorylation and dephosphorylation of protein was studied. cGMP or cAMP with (..gamma..-/sup 32/P)ATP in the dark enhanced the phosphorylation of two ROS proteins with M/sub r/ = 10,500 (Band 1) and 8500 (Band 2) according to sodium dodecylsulfate-polyacrylamide gel electrophoresis analysis. The phosphorylation was maximally enhanced at 2.0 mM cGMP and cAMP in the presence of Mg/sup 2 +/. The cGMP-activated protein kinase showed near-optimal activity between pH 6.5 and 8.0. GMP, GDP, GTP, AMP, and ADP did not enhance the phosphorylation. Both /sup 32/P-phosphorylated Bands 1 and 2 were solubilized during preparation and the molecular weight of each was 19,000. Their isoelectric point was 5.2. The sites of phosphorylation were the serine residue(s). Dephosphorylation of /sup 37/P-Bands 1 and 2 in dark-adapted ROS suspension required Mn/sup 2 +/ or Mg/sup 2 +/. Both phosphorylation and dephosphorylation were inhibited by Zn/sup 2 +/.
- Research Organization:
- Kyoto Univ., Japan
- OSTI ID:
- 5179105
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 261:1
- Country of Publication:
- United States
- Language:
- English
Similar Records
Guanosine 3 prime ,5 prime -cyclic nucleotide binding proteins of bovine retina identified by photoaffinity labeling
Effect of dietary zinc deficiency on the endogenous phosphorylation and dephosphorylation of rat erythrocyte membrane
Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
PHOSPHORYLATION
BIOCHEMICAL REACTION KINETICS
PROTEINS
RETINA
ZINC COMPOUNDS
BIOLOGICAL EFFECTS
AMP
CATIONS
EXPERIMENTAL DATA
FROGS
INHIBITION
LABELLED COMPOUNDS
MAGNESIUM COMPOUNDS
MANGANESE COMPOUNDS
MOLECULAR WEIGHT
NUCLEOTIDES
PHOSPHORUS 32
PHOSPHOTRANSFERASES
SERINE
TRACER TECHNIQUES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHARGED PARTICLES
CHEMICAL REACTIONS
DATA
DAYS LIVING RADIOISOTOPES
ENZYMES
EYES
FACE
HEAD
HYDROXY ACIDS
INFORMATION
IONS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
REACTION KINETICS
SENSE ORGANS
TRANSFERASES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
560305 - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)