Effect of solvent and extraction methods on the bacterial mutagenicity of sidestream cigarette smoke
The mutagenic activity of sidestream cigarette-smoke particles was estimated by testing sidestream cigarette-smoke particles that had been collected under controlled burning conditions in the laboratory. Two different extraction methods (Soxhlet and ultrasonic agitation) and 3 different solvents (dichloromethane, methanol, and acetone) were compared for their efficiencies in the extraction of compounds from sidestream cigarette-smoke particles that are mutagenic in the Ames test. The mutagenic activity of the sidestream smoke particles was estimated to be 15,000-20,000 revertants per cigarette in TA98 with metabolic activation and 12,000-17,000 revertants per cigarette in TA100 without metabolic activation.
- Research Organization:
- Environmental Protection Agency, Research Triangle Park, NC (USA). Health Effects Research Lab.
- OSTI ID:
- 5178159
- Report Number(s):
- PB-88-182381/XAB; EPA-600/J-87/256
- Resource Relation:
- Other Information: Pub. in Toxicology Letters, Vol. 38, 279-290(1987)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AIR POLLUTION
BACTERIA
MUTAGENS
PARTICULATES
MUTAGENESIS
SOLVENT EXTRACTION
TOBACCO SMOKES
ACETONE
GENETICS
LABORATORIES
METABOLISM
SALMONELLA
TOXICITY
AEROSOLS
BIOLOGY
COLLOIDS
DISPERSIONS
EXTRACTION
KETONES
MICROORGANISMS
ORGANIC COMPOUNDS
PARTICLES
POLLUTION
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)