Studies on nerve growth and repair
The transition of nerve cell from a dividing undifferentiated cell to a morphologically differentiated nondividing cell was studied. Pheochromocytoma (PC12) cells respond to nerve growth factor (NGF) by extending electrically excitable neurites. Yet by labeling these cells with /sup 3/H-thymidine and examining them autoradiographically, evidence for continued DNA replication in neurite bearing neurons was found. NGF receptors of two classes are expressed on PC12 cells and other NGF dependent neurons. By labeling differentiated PC12s in culture with /sup 125/I-NGF and examining them autoradiographically both receptor classes were found evenly distributed on the growth cones, neurites and cell bodies. Cytoskeletal associated receptors were found in these areas as well. The purification and identification of a soluble, extracellular protein of 37,000 molecular weight whose synthesis and accumulation is increased after injury of a rat sciatic nerve is described.
- Research Organization:
- Stanford Univ., CA (USA)
- OSTI ID:
- 5177536
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NERVE CELLS
BIOLOGICAL REPAIR
CELL DIFFERENTIATION
AUTORADIOGRAPHY
BIOELECTRICITY
BIOLOGICAL ACCUMULATION
BIOSYNTHESIS
DNA REPLICATION
EXPERIMENTAL DATA
INJURIES
IODINE 125
MOLECULAR WEIGHT
PROTEINS
PURIFICATION
RATS
RECEPTORS
SCIATIC NERVE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL RECOVERY
DATA
DAYS LIVING RADIOISOTOPES
ELECTRICITY
ELECTRON CAPTURE RADIOISOTOPES
HETEROCYCLIC COMPOUNDS
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVES
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RADIOISOTOPES
RECOVERY
REPAIR
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550201 - Biochemistry- Tracer Techniques