Ultralow detection limits for an organic dye determined by fluorescence spectroscopy with laser diode excitation

Journal Article · Sat Apr 15 00:00:00 EDT 1989 · Analytical Chemistry; (USA)

DOI:https://doi.org/10.1021/ac00183a016· OSTI ID:5175204

Johnson, P A; Barber, T E; Smith, B W; Winefordner, J D ^[1]

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1. Univ. of Florida, Gainesville (USA)

Fluorescence of IR-140, a laser dye in methanol solution, is excited by a semiconductor laser diode. Analytical figures of merit are compared for three different instrumental configurations, with the dye measured in a cuvette, a liquid jet, and a compact instrument. The best limit of detection, 46,000 molecules, was achieved with a liquid jet. Linear dynamic range was 6 orders of magnitude. The laser diode operates in the near-infrared region, resulting in low background fluorescence.

• OSTI ID: 5175204
• Journal Information: Analytical Chemistry; (USA), Vol. 61:8; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
42 ENGINEERING
DYES
FLUORESCENCE SPECTROSCOPY
EXPERIMENTAL DATA
JETS
LASER MATERIALS
METHANOL
NEAR INFRARED RADIATION
QUANTITATIVE CHEMICAL ANALYSIS
SEMICONDUCTOR LASERS
SOLUTIONS
TRACE AMOUNTS
ALCOHOLS
CHEMICAL ANALYSIS
DATA
DISPERSIONS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
HYDROXY COMPOUNDS
INFORMATION
INFRARED RADIATION
LASERS
MATERIALS
MIXTURES
NUMERICAL DATA
ORGANIC COMPOUNDS
RADIATIONS
SEMICONDUCTOR DEVICES
SOLID STATE LASERS
SPECTROSCOPY
400102* - Chemical & Spectral Procedures
426002 - Engineering- Lasers & Masers- (1990-)