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Title: Development of a transplantable mouse myeloid leukemia model system: a preliminary report

Abstract

Research progess has been hindered in some areas of carcinogenesis because of a lack of consistant biological markers that distinguish cancer from normal cells. Cell lines derived from x-ray induced RFM mouse myeloid leukemia were studied for the purpose of first identifying the genetic differences (by chromosome analysis) between leukemic and normal cells and second, developing a transplantable leukemia model system. Among the cell lines analyzed, one of them (MLI) appeared to be useful in development of such a model. In addition to two chromosome abnormalities, cell line MLI also has a large abnormal chromosome which may be useful as a biological marker for these leukemic cells. This marker was identified by banding study to be an isochromosome 8 derived from centromeric translocation of two chromosome 8s. Upon injection of 4 x 10/sup 6/ leukemic cells into RFM mice symptoms of leukemia appeared from 18 days onwards and mice would begin to die of leukemia from 21 days onwards. The presence or absence of the metacentric marker chromosome was the criteria for inequivocal identification of leukemic from co-existing normal cells in these two tissues. The ratio of these cell populations was quantitated and the time-dependent increase of the ratios wasmore » indicative of progression of leukemia. Thus, this transplantable myeloid leukemia model system may be useful in studying the proliferation of leukemic cells and the response of the co-existing cells to therapeutic agents. (ERB)« less

Authors:
; ;
Publication Date:
Research Org.:
Oak Ridge National Lab., TN (USA)
OSTI Identifier:
5175186
Report Number(s):
CONF-820439-2
ON: DE82013456
DOE Contract Number:  
W-7405-ENG-26
Resource Type:
Technical Report
Resource Relation:
Conference: Symposium on genetics mechanisms of carcinogenesis, Gatlinburg, TN, USA, 11 Apr 1982
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; MYELOID LEUKEMIA; BIOLOGICAL MODELS; TUMOR CELLS; BIOLOGICAL INDICATORS; BONE MARROW CELLS; CELL CULTURES; CELL PROLIFERATION; CHROMOSOMAL ABERRATIONS; SPLEEN CELLS; TRANSPLANTS; ANIMAL CELLS; CONNECTIVE TISSUE CELLS; DISEASES; HEMIC DISEASES; LEUKEMIA; MUTATIONS; NEOPLASMS; SOMATIC CELLS; 550900* - Pathology; 550300 - Cytology

Citation Formats

Au, W W, Luippold, H E, and Otten, J A. Development of a transplantable mouse myeloid leukemia model system: a preliminary report. United States: N. p., 1982. Web.
Au, W W, Luippold, H E, & Otten, J A. Development of a transplantable mouse myeloid leukemia model system: a preliminary report. United States.
Au, W W, Luippold, H E, and Otten, J A. 1982. "Development of a transplantable mouse myeloid leukemia model system: a preliminary report". United States.
@article{osti_5175186,
title = {Development of a transplantable mouse myeloid leukemia model system: a preliminary report},
author = {Au, W W and Luippold, H E and Otten, J A},
abstractNote = {Research progess has been hindered in some areas of carcinogenesis because of a lack of consistant biological markers that distinguish cancer from normal cells. Cell lines derived from x-ray induced RFM mouse myeloid leukemia were studied for the purpose of first identifying the genetic differences (by chromosome analysis) between leukemic and normal cells and second, developing a transplantable leukemia model system. Among the cell lines analyzed, one of them (MLI) appeared to be useful in development of such a model. In addition to two chromosome abnormalities, cell line MLI also has a large abnormal chromosome which may be useful as a biological marker for these leukemic cells. This marker was identified by banding study to be an isochromosome 8 derived from centromeric translocation of two chromosome 8s. Upon injection of 4 x 10/sup 6/ leukemic cells into RFM mice symptoms of leukemia appeared from 18 days onwards and mice would begin to die of leukemia from 21 days onwards. The presence or absence of the metacentric marker chromosome was the criteria for inequivocal identification of leukemic from co-existing normal cells in these two tissues. The ratio of these cell populations was quantitated and the time-dependent increase of the ratios was indicative of progression of leukemia. Thus, this transplantable myeloid leukemia model system may be useful in studying the proliferation of leukemic cells and the response of the co-existing cells to therapeutic agents. (ERB)},
doi = {},
url = {https://www.osti.gov/biblio/5175186}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}

Technical Report:
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