Molecular mechanism of action of opioids in human neuroblastoma cells

Thesis/Dissertation · Thu Jan 01 00:00:00 EST 1987

OSTI ID:5174907

Yu, V C.K.

A series of human neuroblastoma cell lines was screened for the presence of opioid receptor sites. Of these cell lines, SK-N-SH was found to express approximately 50,000 ..mu.. and 10,000 delta opioid receptor sites/cell. In vitro characterization revealed that the binding properties of these receptor sites closely resembled those of human and rodent brain. Phosphatidylinositol turnover as a potential second messenger system for the ..mu.. receptor was examined in SK-N-SH cells. Neurotransmitter receptor systems were determined in the three sub-clones of SK-N-SH cells. Cells of the SH-SY5Y line, a phenotypically stable subclone of SK-N-SH cells, were induced to differentiate by treatment with various inducing agents, and changes of several neurotransmitter receptor systems were determined. Nerve growth factor (NGF) and retinoic acid (RA) up-regulated, while dBcAMP down-regulated opioid receptor sites. (/sup 3/H)Dopamine uptake was slightly enhanced only in RA-treated cells. Strikingly, the efficacy of PGE/sub 1/-stimulated accumulation of cAMP was enhanced by 15- to 30-fold upon RA treatment.

• Research Organization: California Univ., San Francisco (USA)
• OSTI ID: 5174907
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDORPHINS
BIOCHEMICAL REACTION KINETICS
CHEMICAL PROPERTIES
TUMOR CELLS
CELL DIFFERENTIATION
AMP
DOPAMINE
GROWTH FACTORS
MAN
PHOSPHOLIPIDS
RETINOIC ACID
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
CARBOXYLIC ACID ESTERS
CARDIOTONICS
CARDIOVASCULAR AGENTS
DRUGS
ESTERS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MITOGENS
NEUROREGULATORS
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
REACTION KINETICS
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques