The in vivo fate of a /sup 211/At labelled monoclonal antibody with known specificity in a murine system

Journal Article · Mon Nov 01 00:00:00 EST 1982 · Int. J. Radiat. Oncol., Biol. Phys.; (United States)

DOI:https://doi.org/10.1016/0360-3016(82)90453-9· OSTI ID:5173222

Vaughan, A T.M. ^[1]; Bateman, W J; Fisher, D R

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1. Univ. of Birmingham, England

A monoclonal antibody reactive against the human transferrin receptor has been labelled with the alpha and X ray emitting isotope Astatine 211. The labelling procedure does not affect the ability of the product to bind to the transferrin receptor on the human leukemic cell line HL60. Using a direct binding assay, /sup 211/At labelled antibody can be specifically inhibited from binding to its target cells by excess unlabelled antibody. Furthermore, the binding inhibition demonstrated in this system correlates to enhanced clonogenic survival of these cells, indicating that very few atoms of /sup 211/At/cell are required for cell death. Data obtained from labelled antibody injected into mice show that the labelled product in serum retains the ability to bind to HL60 cells in vitro, although tissue distributions of the injected activity implies that some of the radiolabel is lost from the protein. Despite this loss of label, preliminary experiments on the localization of labelled antibody to HL60 cells growing s/c in nude mice show that tumor tissue has a higher specific activity than all other tissues, other than blood, after 12 hours. This suggests that further work on the nature of label degradation in vivo is warranted in the context of potential therapeutic and diagnostic studies.

• OSTI ID: 5173222
• Journal Information: Int. J. Radiat. Oncol., Biol. Phys.; (United States), Vol. 8:11
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
62 RADIOLOGY AND NUCLEAR MEDICINE
ASTATINE 211
CELL KILLING
MONOCLONAL ANTIBODIES
LABELLING
TRANSFERRIN
RECEPTORS
BIOLOGICAL LOCALIZATION
IMMUNOTHERAPY
IN VITRO
IN VIVO
LABELLED COMPOUNDS
MICE
NEOPLASMS
RADIOIMMUNOLOGY
TRACER TECHNIQUES
ALPHA DECAY RADIOISOTOPES
ANIMALS
ANTIBODIES
ASTATINE ISOTOPES
BETA DECAY RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
GLOBULINS
GLOBULINS-BETA
HEAVY NUCLEI
HOURS LIVING RADIOISOTOPES
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METALLOPROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
RODENTS
THERAPY
VERTEBRATES
560172* - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)
550600 - Medicine