Interactions between macrophage/Kupffer cells and hepatocytes in surgical sepsis
Experiments were performed to investigate the role of Kupffer cell/macrophage interactions with hepatocytes in modulating liver function during infections using direct in vitro cocultivation of rat macrophages or Kupffer cells with rat hepatocytes. Protein synthesis was assayed as a sensitive indicator of integrated hepatocellular function by measuring {sup 3}H-leucine incorporation into hepatocyte protein. Septic stimuli such as lipoploysaccharide and killed bacteria were added to cocultures of hepatocytes and macrophages or Kupffer cells and the responses compared to hepatocytes alone. Information about the types of proteins synthesized by hepatocytes under various culture conditions was determined using polyacrylamide gel electrophoresis and autoradiography. These experiments showed that septic stimuli alter the amount and type of protein synthesized by hepatocytes and had no direct effect on hepatocytes in the absence of macrophages or Kupffer cells. The mediator(s) appears to be a heat labile, soluble monokine(s) which is distinct from interleukin-1 or tumor necrosis factor. The important role of Kupffer cells/macrophages in mediating alterations in hepatocellular function in sepsis may ultimately improve patient care.
- Research Organization:
- Minnesota Univ., Minneapolis, MN (USA)
- OSTI ID:
- 5156936
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BACTERIAL DISEASES
PATHOGENESIS
LIVER CELLS
BIOLOGICAL FUNCTIONS
MACROPHAGES
PROTEINS
BIOSYNTHESIS
AUTORADIOGRAPHY
ELECTROPHORESIS
LEUCINE
RATS
SURGERY
TRITIUM COMPOUNDS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DISEASES
FUNCTIONS
HYDROGEN COMPOUNDS
INFECTIOUS DISEASES
MAMMALS
MEDICINE
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHAGOCYTES
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550901* - Pathology- Tracer Techniques