Control of. cap alpha. -amylase mRNA accumulation by gibberellic acid and calcium in barley aleurone layers
Pulse-labeling of barley (Hordeum vulgare L. cv Himalaya) aleurone layers incubated for 13 hours in 2.5 micromolar gibberellic acid (GA/sub 3/) with or without 5 millimolar CaCl/sub 2/ shows that ..cap alpha..-amylase isozymes 3 and 4 are not synthesized in vivo in the absence of Ca/sup 2 +/. No difference was observed in ..cap alpha..-amylase mRNA levels between layers incubated for 12 hours in 2.5 micromolar GA/sub 3/ with 5 millimolar CaCl/sub 2/ and layers incubated in GA/sub 3/ alone. RNA isolated from layers incubated for 12 hours in GA/sub 3/ with and without CA/sup 2 +/. A cDNA clone for ..cap alpha..-amylase was isolated and used to measure ..cap alpha..-amylase mRNA levels in aleurone layers incubated in the presence and absence of Ca/sup 2 +/ was translated in vitro and was found to produce the same complement of translation products regardless of the presence of Ca/sup 2 +/ in the incubation medium. Immunoprecipitation of translation products showed that the RNA for ..cap alpha..-amylase synthesized in Ca/sup 2 +/-deprived aleurone layers was translatable. Ca/sup 2 +/ is required for the synthesis of ..cap alpha..-amylase isozymes 3 and 4 at a step after mRNA accumulation and processing.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 5151421
- Journal Information:
- Plant Physiol.; (United States), Vol. 78:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AMYLASE
BIOSYNTHESIS
CALCIUM COMPOUNDS
BIOLOGICAL EFFECTS
GIBBERELLIC ACID
BIOCHEMICAL REACTION KINETICS
MESSENGER-RNA
BUILDUP
BARLEY
DNA-CLONING
EXPERIMENTAL DATA
PRECIPITINS
TRACER TECHNIQUES
ALKALINE EARTH METAL COMPOUNDS
ANTIBODIES
CARBOXYLIC ACIDS
CEREALS
CLONING
DATA
ENZYMES
ESTERS
GLYCOSYL HYDROLASES
GRASS
HETEROCYCLIC COMPOUNDS
HYDROLASES
HYDROXY ACIDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LACTONES
NUCLEIC ACIDS
NUMERICAL DATA
O-GLYCOSYL HYDROLASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
RNA
SYNTHESIS
550201* - Biochemistry- Tracer Techniques