Increased activity of 5-lipoxygenase in polymorphonuclear leukocytes from asthmatic patients

Journal Article · Mon Sep 09 00:00:00 EDT 1985 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(85)90526-0· OSTI ID:5149427

Mita, H; Yui, Y; Taniguchi, N; Yasueda, H; Shida, T

The formation of 5-lipoxygenase products of arachidonic acid, 5-HETE and 5,12-diHETE, was determined in 100,000 x g supernatant of polymorphonuclear leukocytes from 17 healthy subjects, 17 patients with extrinsic asthma and 15 patients with intrinsic asthma. After the supernatant was incubated with /sup 14/C-arachidonic acid in the presence of calcium and indomethacin, the lipoxygenase products of arachidonic acid were separated by thin layer chromatography. The results were expressed as the percentage conversion of /sup 14/C-arachidonic acid into the product per 10/sup 7/ cells. The formation of 5,12-diHETE, but not of the 5-HETE, was significantly increased in the cells from the group of patients with extrinsic asthma (4.38 +/- 0.78%, mean +/- S.E.; p < 0.01) and intrinsic asthma (6.09 +/- 1.11%; p < 0.01), when compared to normal subjects (1.74 +/- 0.30%). Both extrinsic and intrinsic asthmatics had significantly enhanced 5-lipoxygenase activity, which was expressed as the sum of percentage conversion of /sup 14/C-arachidonic acid into 5-HETE and 5,12-diHETE. The percentage conversion in normal subjects was 4.19 +/- 0.39%, 6.24 +/- 0.84% for 17 patients with extrinsic asthma (p < 0.05), and 8.59 +/- 1.29% for 15 patients with intrinsic asthma (p < 0.01). There was no significant difference between these asthmatic groups. These results indicate that 5-lipoxygenase activity is increased in patients with bronchial asthma. 22 references, 3 figures.

• Research Organization: National Sagamihara Hospital, Japan
• OSTI ID: 5149427
• Journal Information: Life Sci.; (United States), Vol. 37:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
METABOLISM
OXYGENASES
ENZYME ACTIVITY
ASTHMA
CARBON 14 COMPOUNDS
LEUKOCYTES
PATIENTS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DISEASES
ENZYMES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MATERIALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
RESPIRATORY SYSTEM DISEASES
SEPARATION PROCESSES
550901* - Pathology- Tracer Techniques