Induction of SOS genes of Escherichia coli by chromium compounds
The induction of several SOS genes of Escherichia coli such as recA, umuC, and sfiA by hexavalent (K/sub 2/Cr/sub 2/O/sub 7/, K/sub 2/CrO/sub 4/, and CrO/sub 3/) and trivalent (CrCl/sub 3/, Cr(NO/sub 3/)/sub 3/, and (CH/sub 3/COO)/sub 3/Cr) compounds of chromium was studied. Induction was measured as ..beta..-galactosidase activity, using lacZ gene fusions under the control region of different SOS genes. The hexavalent chromium forms induced the genes responsible for massive synthesis of RecA protein, error-prone repair, and inhibition of cell division. On the other hand, the trivalent chromium compounds were unable to induce any of the SOS genes tested. Individual assay of hexavalent chromium compounds showed that K/sub 2/Cr/sub 2/O/sub 7/ was a stronger inducing agent of those three SOS genes tested than K/sub 2/CrO/sub 4/, which, in turn, was stronger than CrO/sub 3/. All this data led to the conclusion that hexavalent chromium compounds, but not trivalent, are proficient agents of induction of the SOS system and can produce indirect mutagenesis in Escherichia coli.
- Research Organization:
- Autonomous Univ. of Barcelona, Spain
- OSTI ID:
- 5148491
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 8:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMIUM COMPOUNDS
BIOLOGICAL EFFECTS
ESCHERICHIA COLI
ENZYME ACTIVITY
GENES
CHEMICAL ACTIVATION
CHEMICAL STATE
CHROMATES
CHROMIUM CHLORIDES
CHROMIUM NITRATES
ENZYME INDUCTION
GALACTOSIDASE
MUTAGENESIS
BACTERIA
CHLORIDES
CHLORINE COMPOUNDS
ENZYMES
GENE REGULATION
GLYCOSYL HYDROLASES
HALIDES
HALOGEN COMPOUNDS
HYDROLASES
MICROORGANISMS
NITRATES
NITROGEN COMPOUNDS
O-GLYCOSYL HYDROLASES
OXYGEN COMPOUNDS
TRANSITION ELEMENT COMPOUNDS
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)