Enhanced production of cellulase, hemicellulase, and beta-glucosidase by Trichoderma reesei (rut c-30)
The production of cellulases and hemicellulases was studied with Trichoderma reesei Rut C-30. This organism produced, together with high cellulase activities, considerable amounts of xylanases and beta-glucosidase. Three cellulose concentrations (1, 2.5, and 5.0%) were examined to determine the maximum levels of cellulase activity obtainable in submerged culture. Temperature and pH profiling was used to increase cell mass to maximum levels within two days and thereby enhancing fermentor productivity at higher substrate levels. The effect of temperature, pH, Tween-80 concentration, carbon source, and substrate concentration on the ratio of mycelial growth and extracellulose enzyme production are described. 19 refs.
- Research Organization:
- Univ of Calif, Berkeley, USA
- OSTI ID:
- 5135417
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 23:8
- Country of Publication:
- United States
- Language:
- English
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Enhanced production of cellulase, hemicellulase, and b-glucosidase by trichoderma reesei, (rut c-30)
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CELLULASE
BIOSYNTHESIS
ETHANOL
GLUCOSIDASE
TRICHODERMA
CELL CULTURES
CELLULOLYTIC ACTIVITY
XYLANASE
PH VALUE
UREA
ALCOHOLS
AMIDES
CARBONIC ACID DERIVATIVES
ENZYME ACTIVITY
ENZYMES
FUNGI
GLYCOSYL HYDROLASES
HYDROLASES
HYDROXY COMPOUNDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PLANTS
SYNTHESIS
090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)
550200 - Biochemistry
550700 - Microbiology