(Effects of freezing and cold acclimation on the plasma membrane of isolated protoplasts): Progress report, May 16, 1987-January 4, 1988

Technical Report · Fri Jan 01 00:00:00 EST 1988

OSTI ID:5130449

Steponkus, P L

The goals of this project are to provide a mechanistic understanding of freezedehydration-induced mesonmorphic phase transitions in the plasma membrane of winter cereals that are manifested as alterations in the ultrastructure (lateral phase separations, aparticulate lamellae and hexagonal/sub II/ configurations) and which result in the loss of osmotic responsiveness of protoplasts isolated from non-acclimated rye leaves and to determine the cellular and molecular mechanims by which cold acclimation and cryoprotectants preclude or diminish these alternations in the plasma membrane. This past year, our efforts have focused on dehydration-induced lamellar-to-hexagonal/sub II/ phase transitions in the plasma membrane of isolated protoplasts and liposomes of plasma membrane lipids, characterization of the phase behavior plasma membrane lipids, development of a theory for membrane destabilization at low water contents and effects of freezingosmotic dehydration on ATPase activity and protein composition of the plasma membrane.

• Research Organization: Cornell Univ., Ithaca, NY (USA)
• DOE Contract Number: FG02-84ER13214
• OSTI ID: 5130449
• Report Number(s): DOE/ER/13214-2; ON: DE88009095
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
09 BIOMASS FUELS
CELL MEMBRANES
PHASE TRANSFORMATIONS
LIPOSOMES
BIOLOGICAL ADAPTATION
BIOPHYSICS
ELECTRON MICROSCOPY
PLANT CELLS
PROGRESS REPORT
RYE
CELL CONSTITUENTS
CEREALS
DOCUMENT TYPES
GRASS
MEMBRANES
MICROSCOPY
ORGANOIDS
PLANTS
560200* - Thermal Effects
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