Novel Drosophila receptor that binds multiple growth factors
The authors have recently reported the identification of a novel growth factor receptor from Drosophila cell cultures that has dual binding specificity for both insulin and epidermal growth factor (EGF). This 100 kDa protein is also antigenically related to the cytoplasmic region of the mammalian EGF receptor-tyrosine kinase. They now report that this protein binds to mammalian nerve growth factor and human transforming growth factor alpha as well as insulin and EGF with apparent dissociation constants ranging from 10/sup -6/ to 10/sup -8/ M. The 100 kDa protein can be affinity-labeled with these /sup 125/I-labeled growth factors after immunoprecipitation with anti-EGF receptor antiserum. These four growth factors appear to share a common binding site, as evidenced by their ability to block affinity labelling by /sup 125/I-insulin. No significant binding to the 100 kDa protein was observed with platelet-derived growth factor, transforming growth factor beta, or glucagon. The 100 kDa Drosophila protein has a unique ligand-binding spectrum with no direct counterpart in mammalian cells and may represent an evolutionary precursor of the mammalian receptors for these growth factors.
- Research Organization:
- Massachusetts Institute of Technology, Cambridge
- OSTI ID:
- 5127383
- Report Number(s):
- CONF-8606151-; TRN: 86-034804
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
RECEPTORS
BIOCHEMICAL REACTION KINETICS
AFFINITY
DROSOPHILA
INSULIN
IODINE 125
TRACER TECHNIQUES
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DIPTERA
ELECTRON CAPTURE RADIOISOTOPES
FLIES
FRUIT FLIES
HORMONES
INSECTS
INTERMEDIATE MASS NUCLEI
INVERTEBRATES
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HORMONES
RADIOISOTOPES
REACTION KINETICS
550201* - Biochemistry- Tracer Techniques