A cis-active regulatory gene in the mouse: direct demonstration of cis-active control of the rate of enzyme subunit synthesis

Journal Article · Tue Jul 01 00:00:00 EDT 1980 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.77.7.4193· OSTI ID:5127375

Bernstine, E G; Koh, C

Mouse mitochondrial malic enzyme (L-malate:NADP/sup +/ oxidoreductase (oxaloacetate-decarboxylating), EC1.1.1.40) is a tetrameric protein. Two alleles of the structural gene (Mod-2) are known which code for electrophoretically distinct enzyme subunits: Mod-2/sup a/ and Mod-2/sup b/. A regulatory gene (Mdr-1), closely linked to Mod-2 on chromosome 7, determines the rate of mitochondrial malic enzyme synthesis in brain. Two alleles of Mdr-1 are known: Mdr-1/sup a/ (high activity) and Mdr-1/sup b/ (low activity). By pulse-labeling with (/sup 35/S)methionine, immune precipitation, and isoelectric focusing under dissociating conditions, we have measured the relative rates of synthesis of the two types of enzyme subunit in animals of genotypes Mdr-1/sup a/ Mod-2/sup a//Mdr-1/sup a/ Mod-2/sup b/ and Mdr-1/sup a/ Mod-2/sup a//Mdr-1/sup b/ Mod-2/sup b/. The results show that in the former animals both types of subunit are made at an identical rate, whereas in the latter animals the Mod-2/sup a/ gene product is synthesized at a rate 2.2 times that of the Mod-2/sup b/-coded subunit. Thus we have unambiguously demonstrated that Mdr-1 is cis-active in its control of the expression of the Mod-2 structural gene.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5127375
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 77:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENZYME ACTIVITY
GENETIC VARIABILITY
OXIDOREDUCTASES
BIOSYNTHESIS
BRAIN
GENES
GENETICS
GENOTYPE
IMMUNE REACTIONS
LABELLED COMPOUNDS
METHIONINE
MICE
MITOCHONDRIA
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
BIOLOGY
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPOTROPIC FACTORS
MAMMALS
NERVOUS SYSTEM
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANOIDS
ORGANS
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
551001 - Physiological Systems- Tracer Techniques
550401 - Genetics- Tracer Techniques