Flow karyology of serially cultured Chinese hamster cell lineages
Conference
·
· Cytometry (Baltimore); (United States)
OSTI ID:5125903
Flow karyology of serially cultured Chinese hamster cell lineages has been observed to be influenced by the degree of cellular heterogeneity in culture. The minimum coefficient of variation (CV) and debris fraction obtainable vary as a cell lineage evolves from a primary cell culture to an established cell line. The cell lineages pass through a stage of decreased cellular heterogeneity from which flow karyotypes can be obtained with lower CV and debris fraction. The influence of cellular heterogeneity on flow karyology is observed with constant preparative protocol and constant instrument performance, and can be an additional source of variability.
- Research Organization:
- Los Alamos National Lab., NM
- OSTI ID:
- 5125903
- Journal Information:
- Cytometry (Baltimore); (United States), Vol. 5; Conference: Applications of flow cytometry to gene mapping and chromosome analysis, Santa Fe, NM, USA, 23-26 Oct 1983
- Country of Publication:
- United States
- Language:
- English
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