Multiple dispersed spontaneous mutations: A novel pathway of mutation in a malignant human cell line

Journal Article · Sat Jun 01 00:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.6.3163· OSTI ID:5118569

Harwood, J; Tachibana, Akira; Meuth, M ^[1]

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1. Clare Hall Labs., Hertfordshire (United Kingdom)

The authors analyzed the nature of spontaneous mutations at the autosomal locus coding for adenine phosphoribosyltransferase in the human colorectal carcinoma cell line SW620 to establish whether distinctive mutational pathways exist that might underlie the more complex genome rearrangements arising in tumor cells. Point mutations occur at a low rate in part hemizygotes derived from SW620, largely as a result of base substitutions at G {center dot} C base pairs to yield transversions and transitions. However, a novel pathway is evident in the form of multiple dispersed mutations in which two errors, separated by as much as 1,800 bp, fall in the same mutant gene. Such mutations could be the result of error-prone DNA synthesis occurring during normal replication or during long-patch excision-repair of spontaneously arising DNA lesions. This process could also contribute to the chromosomal instability evident in these tumor cells.

• OSTI ID: 5118569
• Journal Information: Molecular and Cellular Biology; (United States), Vol. 11:6; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
SPONTANEOUS MUTATIONS
BIOLOGICAL PATHWAYS
TUMOR CELLS
MOLECULAR BIOLOGY
DNA REPLICATION
MAN
PHOSPHORUS-GROUP TRANSFERASES
ANIMAL CELLS
ANIMALS
ENZYMES
MAMMALS
MUTATIONS
NUCLEIC ACID REPLICATION
ORGANIC COMPOUNDS
PRIMATES
PROTEINS
TRANSFERASES
VERTEBRATES
550200* - Biochemistry