Assessment of cortical and trabecular bone distribution in the beagle skeleton by neutron activation analysis
The distribution of bone calcium between morphologically identifiable cortical and trabecular bone obtained by dissection and quantitated by neutron activation analysis (NAA) is described. The skeleton of a female beagle dog was dissected into approximately 400 pieces and assayed for /sup 49/Ca produced in the University of California, Irvine TRIGA reactor. For each of the skeletal sections, we give the initial weight of the alcohol-fixed tissue, which includes cortical bone, trabecular bone, marrow, and cartilage, and a final tissue weight after the marrow and trabecular bone have been dissected away; total section and cortical section calcium weights are reported. The level of detail is represented, for example, by the vertebrae, which were divided into three parts (body, spine, and transverse processes) and by the long bones, which were divided into 10-12 parts such that characterization of the epiphysis, metaphysis, and diaphysis was accomplished. The median percentage cortical calcium values for cervical, thoracic, and lumbar vertebrae were 82%, 56%, and 66%, respectively; however, variation within these groups and among individual vertebral sections was about a factor of 2. For long bones, the median percentage cortical calcium varied from 90-100% in the midshaft to below 50% in the proximal and distal sections. The final calculated cortical tissue-to-calcium mass ratio (TCR) varied from about 4.5 for midshafts of the long bones to about 9 for thoracic vertebral bodies and indicated that the mineral fraction of cortical bone is not constant throughout the skeleton. The ratio of cortical to trabecular calcium in the skeleton was 79.6:20.4.
- Research Organization:
- Univ. of California, Davis
- OSTI ID:
- 5114839
- Journal Information:
- Anat. Rec.; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CALCIUM
ACTIVATION ANALYSIS
TISSUE DISTRIBUTION
CALCIUM 49
CHEMICAL ANALYSIS
DOGS
NEUTRON REACTIONS
SKELETON
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METALS
ANIMALS
BARYON REACTIONS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CALCIUM ISOTOPES
DISTRIBUTION
ELEMENTS
EVEN-ODD NUCLEI
HADRON REACTIONS
INTERMEDIATE MASS NUCLEI
ISOTOPES
MAMMALS
METALS
MINUTES LIVING RADIOISOTOPES
NUCLEAR REACTIONS
NUCLEI
NUCLEON REACTIONS
ORGANS
RADIOISOTOPES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
400101 - Activation
Nuclear Reaction
Radiometric & Radiochemical Procedures