Autoregulated changes in stability of polyribosome-bound. beta. -tubulin mRNAs are specified by the first 13 translated nucleotides

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.3.1224· OSTI ID:5113890

Yen, T J; Gay, D A; Pachter, J S; Cleveland, D W

The expression of tubulin polypeptides in animal cells is controlled by an autoregulatory mechanism whereby increases in the tubulin subunit concentration result in rapid and specific degradation of tubulin mRNAs. The authors have now determined that the sequences that are necessary and sufficient to specify mouse ..beta..-tubulin mRNAs as substrates for this autoregulated instability reside within the first 13 translated nucleotides (which encode the first four ..beta..-tubulin amino acids Met-Arg-Glu-Ile). This domain has been functionally conserved throughout evolution, inasmuch as sequences isolated from the analogous region of human, chicken, and yeast ..beta..-tubulin mRNAs also confer autoregulation. Further, for an RNA to be a substrate for regulation, not only must it carry the 13-nucleotide coding sequence, but it must also be ribosome bound and its translation must proceed 3' to codon 41.

• Research Organization: Dept. of Biological Chemistry, The Johns Hopkins Univ. School of Medicine, 725 North Wolfe Street, Baltimore, MD (US)
• OSTI ID: 5113890
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MICROTUBULES
GENETIC CONTROL
MESSENGER-RNA
STRUCTURE-ACTIVITY RELATIONSHIPS
AMINO ACID SEQUENCE
ANIMAL CELLS
CHICKENS
CODONS
DNA SEQUENCING
GENE RECOMBINATION PROTEINS
GENE REGULATION
MAN
METABOLISM
PHENOTYPE
RIBOSOMAL RNA
RIBOSOMES
YEASTS
ANIMALS
BIRDS
CELL CONSTITUENTS
CONTROL
FOWL
FUNGI
MAMMALS
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOPROTEINS
ORGANIC COMPOUNDS
ORGANOIDS
PEST CONTROL
PLANTS
PRIMATES
PROTEINS
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550200* - Biochemistry
550400 - Genetics