Specific ganglioside binding to receptor sites on T lymphocytes that couple to ganglioside-induced decrease of CD4 expression

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(89)90123-9· OSTI ID:5102100

Morrison, W J ^[1]; Offner, H ^[2]; Vandenbark, A A ^[3]

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1. Immunology Research Lab., Portland, OR (USA)
2. Immunology Research Lab., OR (USA)
3. Oregon Health Sciences Univ., Portland (USA)

The binding of different gangliosides to rat T-helper lymphocytes was characterized under conditions that decrease CD4 expression on different mammalian T-helper lymphoctyes. Saturation binding by monosialylated ({sub 3}H)-GM{sub 1} to rat T-lymphocytes was time- and temperature-dependent, had a dissociation constant (K{sub D}) of 2.2 {plus minus} 1.4 {mu}M and a binding capacity near 2 fmoles/cell. Competitive inhibition of ({sup 3}H)- GM{sub 1} binding demonstrated a structural-activity related to the number of unconstrained sialic acid moieties on GM{sub 1}-congeneric gangliosides. A comparison between the results of these binding studies and gangliosides-induced decrease of CD4 expression demonstrated that every aspect of ({sup 3}H)-GM{sub 1} binding concurs with ganglioside modulation of CD4 expression. It is concluded that the specific decrease of CD4 expression induced by pretreatment with gangliosides involves the initial process of gangliosides binding to specific sites on CD4{sup {double dagger}} T-helper lymphocytes.

• OSTI ID: 5102100
• Journal Information: Life Sciences; (USA), Vol. 45:14; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GANGLIOSIDES
BIOCHEMICAL REACTION KINETICS
LYMPHOCYTES
RECEPTORS
RATS
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
GLYCOLIPIDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEUKOCYTES
LIPIDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
ORGANIC COMPOUNDS
PROTEINS
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques