Energy dispersive X-ray microanalysis, neutron activation analysis and atomic absorption spectrometry--comparison using biological specimens

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Scanning Miscroscopy; (USA)

OSTI ID:5100010

Wroblewski, R; Wroblewski, J; Lundstroem, H E; Edstroem, L J; Jansson, E ^[1]

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1. St Goeran Hospital, Stockholm (Sweden)

X-ray microanalysis, neutron activation analysis and atomic absorption spectrometry were performed on normal and injured skeletal muscle. X-ray microanalysis of tenotomized rat soleus muscle showed significantly elevated levels of sodium and chlorine and lower potassium compared with normal muscle. Similar ion shifts could be demonstrated by neutron activation analysis and atomic absorption spectrometry. The concentrations of sodium and chlorine obtained by these techniques were somewhat higher and that of potassium lower than the values obtained by X-ray microanalysis. This can probably be attributed to the fact that in atomic absorption spectrometry and in neutron activation analysis the entire muscle biopsy contents are measured while in X-ray microanalysis only the content of muscle cells unaffected by extracellular, non-muscular components are determined. It can be concluded that X-ray microanalysis is a reliable technique to study the elemental content of biological tissue, especially tissue undergoing pathological changes affecting the extracellular spaces. Other types of analysis should be used when elements not detectable by X-ray microanalysis are of interest.

• OSTI ID: 5100010
• Journal Information: Scanning Miscroscopy; (USA), Vol. 3:2; ISSN 0891-7035
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MUSCLES
CHEMICAL ANALYSIS
ABSORPTION SPECTRA
ACTIVATION ANALYSIS
BIOPSY
CHLORIDES
COMPARATIVE EVALUATIONS
ELECTRON MICROPROBE ANALYSIS
INJURIES
NEUTRON REACTIONS
POTASSIUM COMPOUNDS
RATS
SODIUM COMPOUNDS
SPECTROPHOTOMETRY
ALKALI METAL COMPOUNDS
ANIMALS
BARYON REACTIONS
CHLORINE COMPOUNDS
DIAGNOSTIC TECHNIQUES
HADRON REACTIONS
HALIDES
HALOGEN COMPOUNDS
MAMMALS
MICROANALYSIS
NUCLEAR REACTIONS
NUCLEON REACTIONS
RODENTS
SPECTRA
VERTEBRATES
550901* - Pathology- Tracer Techniques