Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB

Journal Article · Sun Oct 01 00:00:00 EDT 1989 · American Journal of Surgery; (USA)

DOI:https://doi.org/10.1016/0002-9610(89)90131-1· OSTI ID:5099178

Mustoe, T A; Purdy, J; Gramates, P; Deuel, T F; Thomason, A; Pierce, G F ^[1]

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1. Washington Univ. Medical Center, St. Louis, MO (USA)

This study examined the potential influence of platelet-derived growth factor-BB homodimers (PDGF-BB) on surgical incisions in irradiated animals with depressed wound healing. Rats were irradiated with either 800 rads total body or 2,500 rads surface irradiation. Parallel dorsal skin incisions were made 2 days later, and PDGF-BB was applied topically a single time to one of two incisions. In total body-irradiated rats, bone marrow-derived elements were severely depressed, wound macrophages were virtually eliminated, and PDGF-BB treatment was ineffective. However, in surface-irradiated rats, PDGF-BB treatment recruited macrophages into wounds and partially reversed impaired healing on day 7 (p less than 0.005) and day 12 (p less than 0.001). PDGF-BB-treated wounds were 50 percent stronger than the paired control wounds. The results suggest PDGF requires bone marrow-derived cells, likely wound macrophages, for activity and that it may be useful as a topical agent in postirradiation surgical incisions.

• OSTI ID: 5099178
• Journal Information: American Journal of Surgery; (USA), Vol. 158:4; ISSN 0002-9610
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
GROWTH FACTORS
BIOLOGICAL FUNCTIONS
WOUNDS
HEALING
BONE MARROW
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
MACROPHAGES
PROTEINS
RADIOTHERAPY
RATS
SKIN
WHOLE-BODY IRRADIATION
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BIOLOGICAL RECOVERY
BODY
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
FUNCTIONS
HEMATOPOIETIC SYSTEM
INJURIES
IRRADIATION
MAMMALS
MEDICINE
MITOGENS
NUCLEAR MEDICINE
ORGANIC COMPOUNDS
ORGANS
PHAGOCYTES
RADIOLOGY
RECOVERY
RODENTS
SOMATIC CELLS
THERAPY
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals