Detection of an azido-(/sup 14/C)-atrazine labeled protein transferred to nitrocellulose paper

Conference · Tue Apr 01 00:00:00 EST 1986 · Plant Physiol., Suppl.; (United States)

OSTI ID:5098188

Ivey, S; Metz, J G; Berg, S P

An electrophoretically similar protein in spinach and maize can be covalently labeled with azido-(/sup 14/C)-atrazine and separated by 10-18% gradient LDS-PAGE. The protein profile can be transferred to nitrocellulose paper (ncp) by western blotting. The ncp containing the protein profile is sliced into 2 mm slices and counted with liquid scintillation. The labeled protein migrates as a diffuse band with a Mr of 34 kD. This band migrates at a higher Mr (40 kD) under different gel conditions. The ncp dissolves in the organic scintillation cocktail thus providing a more sensitive and quantitative detection of the /sup 14/C. This technique allows the simultaneous immunological and radiochemical identification of many electrophoretically separable proteins.

• Research Organization: Dept. of Biological Sciences, Denver, CO
• OSTI ID: 5098188
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986; Other Information: C45.00*
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MAIZE
PROTEINS
LABELLING
SPINACH
CARBON 14 COMPOUNDS
ELECTROPHORESIS
NITROCELLULOSE
SEPARATION PROCESSES
CARBOHYDRATES
CELLULOSE ESTERS
CEREALS
CHEMICAL EXPLOSIVES
ESTERS
EXPLOSIVES
FOOD
GRASS
LABELLED COMPOUNDS
NITRIC ACID ESTERS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
VEGETABLES
550200* - Biochemistry