Convergent transcription initiates from oppositely oriented promoters within the 5 prime end regions of Drosophila melanogaster F elements

Journal Article · Tue Oct 01 00:00:00 EDT 1991 · Molecular and Cellular Biology; (United States)

DOI:https://doi.org/10.1128/MCB.11.10.5171· OSTI ID:5089922

Minchiotti, G ^[1]; Di Nocera, P P ^[2]

---

1. Consiglio Nazionale Delle Ricerche, Naples (Italy)
2. Univ. di Lecce (Italy)

Drosophila melanogaster F elements are mobile, oligo(A)-terminated DNA sequences that likely propagate by the retrotranscription of RNA intermediates. Plasmids bearing DNA segments from the left-hand region of a full-length F element fused to the CAT gene were used as templates for transient expression assays in Drosophila Schneider II cultured cells. Protein and RNA analyses led to the identification of two promoters, F{sub in} and F{sub out}, that transcribe in opposite orientations. Analysis of the template activity of 3{prime} deletion derivatives indicates that the level of accumulation of F{sub in}RNA is also dependent upon the presence of sequences located within the +175 to +218 interval. The F{sub out} promoter drives transcription in the opposite orientation with respect to F{sub in}, F{sub out} transcripts initiate at nearby sites within the +92 to +102 interval. Sequences downstream of these multiple RNA start sites are not required for the activity of the F{sub out} promoter. Deletions knocking out the F{sub in} promoter do not impair F{sub out} transcription; conversely, initiation at the F{sub in} promoter still takes place in templates that lack the F{sub out} promoter. At a low level, both promoters are active in cultured cells.

• OSTI ID: 5089922
• Journal Information: Molecular and Cellular Biology; (United States), Vol. 11:10; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENE REPRESSORS
DNA SEQUENCING
TRANSCRIPTION
MOLECULAR BIOLOGY
CELL CULTURES
DROSOPHILA
MESSENGER-RNA
OLIGONUCLEOTIDES
PLASMIDS
ANIMALS
ARTHROPODS
CELL CONSTITUENTS
DIPTERA
FLIES
FRUIT FLIES
INSECTS
INVERTEBRATES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RNA
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry