Structure, sequence, expression, and chromosomal localization of the human V{sub 1a} vasopressin receptor gene
- Case Western Reserve Univ. School of Medicine, Cleveland, OH (United States); and others
We recently reported the structure and functional expression of a human V{sub 1a} vasopressin receptor (V{sub 1a}R) cDNA isolated from human liver cDNA libraries. To understand further the expression and regulation of the V{sub 1a}R, we now describe the genomic characteristics, tissue expression, chromosomal localization, and regional mapping of the human V{sub 1a}R gene, AVPR1A. Tissue distribution of the human V{sub 1a}R mRNA explored by Northern blot analysis of various human tissues or organs revealed the presence of a 5.5-kb mRNA transcript expressed in the liver and to a lesser degree in the heart, the kidney, and skeletal muscle. Screening of human genomic libraries revealed that the human AVPR1A gene is included entirely within a 6.4-kb rated by a 2.2-kb intron located before the corresponding seventh transmembrane domain of the receptor sequence. The first exon also contains 2 kb of 5{prime}-untranslated region, and the second exon includes 1 kb of 3{prime}-untranslated region. 5{prime}-RACE analysis of human liver mRNA by PCR localized the V{sub 1a}R mRNA transcription start site 1973 bp upstream of the translation the intron sequence were used as primers in polymerase chain reaction (PCR) analysis of human/rodent somatic cell hybrids. AVPR1A was localized by PCR analysis of a somatic cell hybrid panel to chromosome 12. Fluorescence in situ hybridization using a yeast artificial chromosome physically mapped AVPR1A to region 12q14-q15. 34 refs., 4 figs.
- OSTI ID:
- 508332
- Journal Information:
- Genomics, Vol. 31, Issue 3; Other Information: PBD: 1 Feb 1996
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BASIC STUDIES
HUMAN CHROMOSOME 12
GENETIC MAPPING
VASOPRESSIN
RECEPTORS
STRUCTURE-ACTIVITY RELATIONSHIPS
DNA SEQUENCING
GENE REGULATION
TISSUE DISTRIBUTION
TRANSCRIPTION
DNA-CLONING
OLIGONUCLEOTIDES
SOMATIC CELLS
HYBRIDIZATION
MESSENGER-RNA
POLYMERASE CHAIN REACTION
DNA HYBRIDIZATION
FLUORESCENCE
PROBES