Cloning and expression of a cDNA encoding human sterol carrier protein 2

Journal Article · Tue Jan 15 00:00:00 EST 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.2.463· OSTI ID:5079478

Yamamoto, Ritsu; Kallen, C B; Babalola, G O; Rennert, H; Strauss, III, J F ^[1]; Billheimer, J T ^[2]

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1. Univ. of Pennsylvania School of Medicine, Philadelphia (United States)
2. E.I. DuPont de Nemours, Inc., Wilmington, DE (United States)

The authors report the cloning and expression of a cDNA encoding human sterol carrier protein 2 (SCP{sub 2}). The 1.3-kilobase (kb) cDNA contains an open reading frame which encompasses a 143-amino acid sequence which is 89% identical to the rat SCP{sub 2} amino acid sequence. The deduced amino acid sequence of the polypeptide reveals a 20-residue amino-terminal leader sequence in front of the mature polypeptide, which contains a carboxyl-terminal tripeptide (Ala-Lys-Leu) related to the peroxisome targeting sequence. The expressed cDNA in COS-7 cells yields a 15.3-kDa polypeptide and increased amounts of a 13.2-kDa polypeptide, both reacting with a specific rabbit antiserum to rat liver SCP{sub 2}. The cDNA insert hybridizes with 3.2- and 1.8-kb mRNA species in human liver poly(A){sup +} RNA. In human fibroblasts and placenta the 1.8-kb mRNA was most abundant. Southern blot analysis suggests either that there are multiple copies of the SCP{sub 2} gene in the human genome or that the SCP{sub 2} gene is very large. Coexpression of the SCP{sub 2} cDNA with expression vectors for cholesterol side-chain cleavage enzyme and adrenodoxin resulted in a 2.5-fold enhancement of progestin synthesis over that obtained with expression of the steroidogenic enzyme system alone. These findings are concordant with the notion that SCP{sub 2} plays a role in regulating steroidogenesis, among other possible functions.

• OSTI ID: 5079478
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:2; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PROTEINS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
STEROLS
MASS TRANSFER
DNA-CLONING
GENE REGULATION
LIVER
LIVER CELLS
MAN
ANIMAL CELLS
ANIMALS
BODY
CLONING
DIGESTIVE SYSTEM
DNA
DNA HYBRIDIZATION
GLANDS
HYBRIDIZATION
HYDROXY COMPOUNDS
MAMMALS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PRIMATES
SOMATIC CELLS
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques