Differential scanning calorimetry of plant cell walls
- Washington Univ., St. Louis, MO (United States)
- Monsanto Co., St. Louis, MO (United States)
High-sensitivity differential scanning calorimetry has been used to study the phase transition of cell wall preparations of the elongating and mature regions of soybean hypocotyls and of celery epidermis and collenchyma strands. A step-like transition believed to be glass transition was observed in walls isolated from the elongating region of soybean hypocotyls at 52.9C. Addition of 1 mM CaCl{sub 2} to the cell wall preparation increased the transition temperature to 60.8C and greatly reduced the transition magnitude. In walls from the mature region, the transition was small and occurred at a higher temperature (60.1C). Addition of calcium to the mature region cell wall had little effect on the transition. Based on the known interactions between calcium and pectin, the authors propose that calcium affects the glass transition by binding to the polygalacturonate backbone of wall pectin, resulting in a more rigid wall with a smaller transition at a higher temperature. The mature region either has more calcium in the wall or has more methyl-esterified pectin, making it less responsive to added calcium.
- DOE Contract Number:
- FG02-84ER13255
- OSTI ID:
- 5078163
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:6; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL WALL
PHASE STUDIES
PLANT CELLS
DIFFERENTIAL THERMAL ANALYSIS
CALCIUM CHLORIDES
CALORIMETRY
PECTINS
SOYBEANS
ALKALINE EARTH METAL COMPOUNDS
BLOOD SUBSTITUTES
CALCIUM COMPOUNDS
CALCIUM HALIDES
CARBOHYDRATES
CELL CONSTITUENTS
CHLORIDES
CHLORINE COMPOUNDS
DRUGS
FOOD
HALIDES
HALOGEN COMPOUNDS
HEMATOLOGIC AGENTS
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
THERMAL ANALYSIS
VEGETABLES
550300* - Cytology