Crystal structure of interleukin 8: Symbiosis of NMR and crystallography
Abstract
The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 {angstrom} x-ray data to an R factor of 0.817. A comparison indicates some potential differences between the structure in solution and in the crystalline state. The analysis also predicts that residues 4 through 9 on the amino terminus and the {beta}-bend, which includes His-33, may be important for receptor binding.
- Authors:
-
- National Cancer Inst., Frederick, MD (United States)
- National Inst. of Health, Bethesda, MD (United States)
- Dainippon Pharmaceutical Co., Ltd., Osaka (Japan)
- Wayne State Univ. School of Medicine, Detroit, MI (United States)
- Publication Date:
- OSTI Identifier:
- 5076084
- Resource Type:
- Journal Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America; (United States)
- Additional Journal Information:
- Journal Volume: 88:2; Journal ID: ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 62 RADIOLOGY AND NUCLEAR MEDICINE; GROWTH FACTORS; X-RAY DIFFRACTION; LYMPHOKINES; NUCLEAR MAGNETIC RESONANCE; AQUEOUS SOLUTIONS; CRYSTALLOGRAPHY; MAN; MONOCYTES; RECEPTORS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; COHERENT SCATTERING; DIFFRACTION; DISPERSIONS; LEUKOCYTES; MAGNETIC RESONANCE; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MITOGENS; MIXTURES; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RESONANCE; SCATTERING; SOLUTIONS; VERTEBRATES; 550602* - Medicine- External Radiation in Diagnostics- (1980-)
Citation Formats
Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, and Edwards, B F.P. Crystal structure of interleukin 8: Symbiosis of NMR and crystallography. United States: N. p., 1991.
Web. doi:10.1073/pnas.88.2.502.
Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, & Edwards, B F.P. Crystal structure of interleukin 8: Symbiosis of NMR and crystallography. United States. https://doi.org/10.1073/pnas.88.2.502
Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, and Edwards, B F.P. 1991.
"Crystal structure of interleukin 8: Symbiosis of NMR and crystallography". United States. https://doi.org/10.1073/pnas.88.2.502.
@article{osti_5076084,
title = {Crystal structure of interleukin 8: Symbiosis of NMR and crystallography},
author = {Baldwin, E T and Weber, I T and Charles, R and Xuan, Jiancheng and Matsushima, Kouji and Wlodawer, A and Appella, E and Clore, G M and Gronenborn, A M and Yamada, Masaki and Edwards, B F.P.},
abstractNote = {The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 {angstrom} x-ray data to an R factor of 0.817. A comparison indicates some potential differences between the structure in solution and in the crystalline state. The analysis also predicts that residues 4 through 9 on the amino terminus and the {beta}-bend, which includes His-33, may be important for receptor binding.},
doi = {10.1073/pnas.88.2.502},
url = {https://www.osti.gov/biblio/5076084},
journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:2,
place = {United States},
year = {Tue Jan 15 00:00:00 EST 1991},
month = {Tue Jan 15 00:00:00 EST 1991}
}
Other availability
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.