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Title: Crystal structure of interleukin 8: Symbiosis of NMR and crystallography

Abstract

The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 {angstrom} x-ray data to an R factor of 0.817. A comparison indicates some potential differences between the structure in solution and in the crystalline state. The analysis also predicts that residues 4 through 9 on the amino terminus and the {beta}-bend, which includes His-33, may be important for receptor binding.

Authors:
; ; ; ; ;  [1]; ; ;  [2];  [3];  [4]
  1. National Cancer Inst., Frederick, MD (United States)
  2. National Inst. of Health, Bethesda, MD (United States)
  3. Dainippon Pharmaceutical Co., Ltd., Osaka (Japan)
  4. Wayne State Univ. School of Medicine, Detroit, MI (United States)
Publication Date:
OSTI Identifier:
5076084
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 88:2; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; GROWTH FACTORS; X-RAY DIFFRACTION; LYMPHOKINES; NUCLEAR MAGNETIC RESONANCE; AQUEOUS SOLUTIONS; CRYSTALLOGRAPHY; MAN; MONOCYTES; RECEPTORS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; COHERENT SCATTERING; DIFFRACTION; DISPERSIONS; LEUKOCYTES; MAGNETIC RESONANCE; MAMMALS; MATERIALS; MEMBRANE PROTEINS; MITOGENS; MIXTURES; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RESONANCE; SCATTERING; SOLUTIONS; VERTEBRATES; 550602* - Medicine- External Radiation in Diagnostics- (1980-)

Citation Formats

Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, and Edwards, B F.P. Crystal structure of interleukin 8: Symbiosis of NMR and crystallography. United States: N. p., 1991. Web. doi:10.1073/pnas.88.2.502.
Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, & Edwards, B F.P. Crystal structure of interleukin 8: Symbiosis of NMR and crystallography. United States. https://doi.org/10.1073/pnas.88.2.502
Baldwin, E T, Weber, I T, Charles, R, Xuan, Jiancheng, Matsushima, Kouji, Wlodawer, A, Appella, E, Clore, G M, Gronenborn, A M, Yamada, Masaki, and Edwards, B F.P. 1991. "Crystal structure of interleukin 8: Symbiosis of NMR and crystallography". United States. https://doi.org/10.1073/pnas.88.2.502.
@article{osti_5076084,
title = {Crystal structure of interleukin 8: Symbiosis of NMR and crystallography},
author = {Baldwin, E T and Weber, I T and Charles, R and Xuan, Jiancheng and Matsushima, Kouji and Wlodawer, A and Appella, E and Clore, G M and Gronenborn, A M and Yamada, Masaki and Edwards, B F.P.},
abstractNote = {The crystal structure of a host defense system chemotactic factor, interleukin 8, has been solved by molecular replacement using as a model the solution structure derived from nuclear magnetic resonance experiments. The structure was refined with 2 {angstrom} x-ray data to an R factor of 0.817. A comparison indicates some potential differences between the structure in solution and in the crystalline state. The analysis also predicts that residues 4 through 9 on the amino terminus and the {beta}-bend, which includes His-33, may be important for receptor binding.},
doi = {10.1073/pnas.88.2.502},
url = {https://www.osti.gov/biblio/5076084}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 88:2,
place = {United States},
year = {Tue Jan 15 00:00:00 EST 1991},
month = {Tue Jan 15 00:00:00 EST 1991}
}