Double label autoradiography--an improvement
Journal Article
·
· J. Histochem. Cytochem.; (United States)
A convenient method for double-label autoradiography is described that uses an aqueous mountant, Gelutol (polyvinyl alcohol), which keeps the gelatin spacer in the final autoradiograph permanently swollen to a thickness of around 18 microns in contrast to its 5 microns thickness during exposure of the autoradiograph. This greatly improves optical discrimination between upper and lower layers without the loss of sensitivity or resolution that would result if a 18 microns spacer were used during exposure.
- Research Organization:
- Univ. of Colorado, Boulder, CO (United States)
- OSTI ID:
- 5073447
- Journal Information:
- J. Histochem. Cytochem.; (United States), Vol. 10
- Country of Publication:
- United States
- Language:
- English
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