Genetic-molecular basis for a simple Drosophila melanogaster somatic system that detects environmental mutagens

Journal Article · Mon Sep 01 00:00:00 EDT 1986 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.83.18.6667· OSTI ID:5071231

Green, M M; Todo, T; Ryo, H; Fujikawa, K

We have developed a simple, objectively scorable test for the mutagenicity of chemical compounds which can be fed Drosophila melanogaster. The test depends upon the somatic reversion of the X chromosome, recessive eye color mutation, white-ivory (wi) to wild type (w+). Reversions are scored as clones of w+ facets in the wi eyes of eclosing adults. To increase the sensitivity, a tandem quadruplication containing four wi mutations was synthesized. Thus, in homozygous females eight wi mutations are potentially revertible. Six mutagenic compounds, all alkylating agents, all gave positive results at several concentrations tested. Molecular analysis demonstrates that the induced reversions, germinal and somatic, are associated with the loss of 2.9-kilobase DNA duplicated in the wi mutation.

• Research Organization: Osaka Univ., Japan
• OSTI ID: 5071231
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 18
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
POLLUTANTS
MUTAGEN SCREENING
DROSOPHILA
GENETIC EFFECTS
MUTAGENS
MUTATIONS
PHENOTYPE
X CHROMOSOME
ANIMALS
ARTHROPODS
BIOLOGICAL EFFECTS
CHROMOSOMES
DIPTERA
FLIES
FRUIT FLIES
HETEROCHROMOSOMES
INSECTS
INVERTEBRATES
SCREENING
560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)